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. 2022 Jun 20;13:3518. doi: 10.1038/s41467-022-31268-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic illustration of MetTracer workflow. b Detailed data processing workflow in MetTracer. c Distributions of the 830 labeled metabolites in 293T cells in the metabolic network (left panel) and pathways (right panel). Red dots in the left panel represent the labeled metabolites. The circle size in the right panel represents the ratio of the number of labeled metabolites to the number of metabolites in a pathway. d Numbers of labeled metabolites and isotopologues using MetTracer and other indicated software tools. The Venn diagram shows the overlap of the labeled metabolites using MetTracer and other software. e Distribution of relative errors of labeling extent values between MetTracer and manual analysis using Skyline (n = 830 metabolites). f Relative standard deviation (RSD) distributions of metabolites and isotopologues obtained from MetTracer and other software tools (n = 6 technical replicates of 293T cell samples). The black dots represent median RSD. g False-positive rates of the labeled metabolites and isotopologues obtained from MetTracer and El-MAVEN. Source data are provided as a Source Data file.