Fig. 4. 3-Deazaadenosine, an inhibitor of m⁶A, reduces DENV replication in Ae. aegypti cells.

a Confirmation of the effect of 3-DAA on inhibition of m⁶A modification in Aag2 cells. Total RNA from 3-DAA treated Aag2 and control cells (DMSO treated) were extracted and subjected to a dot blot assay using a specific anti-m⁶A antibody. The input RNAs were directly stained with ethidium bromide. b Inhibition of DENV replication by 3-DAA. Aag2 cells treated with 100 μM 3-DAA or DMSO were infected with DENV 72 h after treatment with 3-DAA. Cells were collected at 72 hpi for quantification of DENV RNA by RT-qPCR. c Viability of Aag2 cells treated with 3-DAA (100 μM) measured at 72 hpi. Error bars represent mean ± S.D. ns, not significant; *p < 0.05, t-test.