Fig. 7. Ubc9 facilitates DENV replication in Ae. aegypti cells.
a Confirmation of silencing of four genes selected from differentially expressed genes by RNAi in Aag2 cells. b Inhibition of DENV replication by Ubc9 (AAEL025779) silencing. Aag2 cells transfected with dsRNA targeting GFP or dsRNA targeting the four genes were infected with DENV-2 72 h after transfection. Cells were collected at 72 hpi for quantification of DENV RNA by RT-qPCR. Control GFP expression levels in a and b were adjusted to 1. ***p < 0.001 (t-test). c Location of the m6A peak in Ubc9. d RT-qPCR analysis of RNA from Aag2 cells infected with DENV for 72 h showed consistency in up-regulation of Ubc9 transcript levels with the RNA-Seq data. FC, fold change. e Aag2 cells transfected with pSLfa-GFP or pSLfa-GFP-Ubc9 were infected with DENV 72 h after transfection. Cells were collected at 72 hpi for RNA extraction and quantification of DENV genomic RNA by RT-qPCR. Error bars represent mean ± S.D. *p < 0.05; **p < 0.01. One-way ANOVA with post hoc comparisons.