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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Dev Neurosci. 2021 Dec 21;44(4-5):214–232. doi: 10.1159/000521611

Figure 7. Disturbed PNN formation in the dorsal hippocampus of IUGR mice at P40.

Figure 7.

Analysis performed in z-stacks captured from fluorescein conjugated WFA staining detecting PNN in green channel in dorsal hippocampus. Box and whiskers plots represent PNN number per mm2 in the dorsal hippocampus and the CA3 stratified by sex (A1), total volume (in μm3 × 103 μm3; A2) and total WFA IF (A.U. × 103 μm3; A3) at P40 in CA1 and CA3. Sexual dimorphism for total PNN volume (A2’) and WFA IF (A3’) are also shown. Boxes are limited by the 75th and 25th percentiles (interquartile range, IQR) and whiskers are limited by the last data point within 1.5 times the IQR from the median (continuous line inside the box). Mann-Whitney U test was applied to compared experimental groups. *, p < 0.05. Unbiased image processing and analysis was performed using Imaris x64 v9.8 software blinded to treatments, sex and time.

Representative surface reconstruction renderings from z-stacks using Zen blue software in CA1 (B1 – B2) and CA3 (B3 – B4), showing reconstructed PNNs (green channel). Videos capturing 3D-reconstructions are available on-line (Supplemental material). Number of NPTX2+ puncta per 103 μm3 (C1) and number of NPTX2+ puncta per 102 μm of PV IN surface area (C2) (y-axis), both directly correlated with WFA IF per PNN (x-axis) at P40 in the CA1 and CA3. Continuous line represents the fitted line derived from a linear regression and the discontinuous line represent the 95% CI. Spearman Rho correlation was applied. A.U., arbitrary units; CA, cornus ammonis; IF, immunofluorescence; NPTX2, neuronal pentraxin 2; P, post-natal age; PNN, perineural nets; PV, parvalbumin; SA, surface area; TXA, thromboxane A2-analog; WFA, Wisteria floribunda lectin.