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Detection of Ply in cell extracts and supernatants of recombinant L. lactis cultured for 12 h by immunoblotting with Ply-specific antibodies. Lanes 1 and 2, cell extract and supernatant fraction (respectively) of L. lactis MG1363(pLC-PL511); lanes 3 and 4, cell extract and supernatant fraction (respectively) of MG1363(pSL-PL511); lane 5, supernatant of MG1363(pSL-PL511ΔC). The positions of molecular mass markers (in kilodaltons) are indicated on the left.
