TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strain | ||
| E. coli | ||
| XL1-Blue MRF′ | Cloning host: Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacZqΔM15, Tn10(Tetr)] | Stratagene |
| DH5α MCR | Cloning host: F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 supE44 λ− thi-1 gyrA96 relA1 | Life Technologies |
| L. lactis | ||
| MG1363 | Plasmid-free derivative of SH4109, Lac− Prt− | 9 |
| Wg2 | Industrial starter strain | 28 |
| Bu2-129 | Lac+ Cit− | 26 |
| L. brevis DSM/20556 | Same as ATCC 8287 | DSM |
| L. monocytogenes WSLC 1001 | Same as ATCC 19112, serovar 1/2c | Weihenstephan Listeria Collection |
| Plasmid | ||
| pBluescript II SK(−) | Cloning and expression vector; AmprlacZ | Stratagene |
| pTRKH2 | Shuttle cloning vector; Emr, lac, gram-negative ori-p15A, gram-positive ori-pAMβ1, high copy number in Lactococcus spp. | 27 |
| pBPL118 | ply118 inserted into the EcoRI/SalI site of pBluescript II SK(−) | This study |
| pBPL118-P21 | Promoter P21 inserted into the XbaI/BamHI site of pBPL118 | This study |
| pBPL118-P32 | Promoter P32 inserted into the XbaI/BamHI site of pBPL118 | This study |
| pBPL118-P59 | Promoter P59 inserted into the XbaI/BamHI site of pBPL118 | This study |
| pLC-PL118-P21 | P21–ply118 cassette from pBPL118-P21 inserted into the XbaI/SalI site of pTRKH2 | This study |
| pLC-PL118-P32 | P32–ply118 cassette from pBPL118-P32 inserted into the XbaI/SalI site of pTRKH2 | This study |
| pLC-PL118-P59 | P59–ply118 cassette from pBPL118-P59 inserted into the XbaI/SalI site of pTRKH2 | This study |
| pLC-PL511 | ply511 inserted into the PstI/SalI site of pLC-PL118-P32, replacing ply118 | This study |
| pSL-ΔSPNuc | SPslpA-ΔSPnuc fusion inserted into PstI/SalI digested pLC-PL118-P32, replacing ply118 | This study |
| pSL-PL118 | SPslpA–ply118 fusion inserted into PstI/SalI digested pLC-PL118-P32, replacing ply118 | This study |
| pSL-PL511 | SPslpA–ply511 fusion inserted into PstI/SalI digested pLC-PL118-P32, replacing ply118 | This study |
| pSL-PL511ΔC | pSL-PL511 encoding C terminally truncated SPSlpA-Ply511Δ(S294–K373) | This study |