Strain |
E. coli
|
XL1-Blue MRF′ |
Cloning host: Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacZqΔM15, Tn10(Tetr)] |
Stratagene |
DH5α MCR |
Cloning host: F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 supE44 λ− thi-1 gyrA96 relA1
|
Life Technologies |
L. lactis
|
MG1363 |
Plasmid-free derivative of SH4109, Lac− Prt−
|
9 |
Wg2 |
Industrial starter strain |
28 |
Bu2-129 |
Lac+ Cit−
|
26 |
L. brevis DSM/20556 |
Same as ATCC 8287 |
DSM |
L. monocytogenes WSLC 1001 |
Same as ATCC 19112, serovar 1/2c |
Weihenstephan Listeria Collection |
Plasmid |
pBluescript II SK(−) |
Cloning and expression vector; AmprlacZ
|
Stratagene |
pTRKH2 |
Shuttle cloning vector; Emr, lac, gram-negative ori-p15A, gram-positive ori-pAMβ1, high copy number in Lactococcus spp. |
27 |
pBPL118 |
ply118 inserted into the EcoRI/SalI site of pBluescript II SK(−) |
This study |
pBPL118-P21 |
Promoter P21 inserted into the XbaI/BamHI site of pBPL118 |
This study |
pBPL118-P32 |
Promoter P32 inserted into the XbaI/BamHI site of pBPL118 |
This study |
pBPL118-P59 |
Promoter P59 inserted into the XbaI/BamHI site of pBPL118 |
This study |
pLC-PL118-P21 |
P21–ply118 cassette from pBPL118-P21 inserted into the XbaI/SalI site of pTRKH2 |
This study |
pLC-PL118-P32 |
P32–ply118 cassette from pBPL118-P32 inserted into the XbaI/SalI site of pTRKH2 |
This study |
pLC-PL118-P59 |
P59–ply118 cassette from pBPL118-P59 inserted into the XbaI/SalI site of pTRKH2 |
This study |
pLC-PL511 |
ply511 inserted into the PstI/SalI site of pLC-PL118-P32, replacing ply118
|
This study |
pSL-ΔSPNuc |
SPslpA-ΔSPnuc fusion inserted into PstI/SalI digested pLC-PL118-P32, replacing ply118
|
This study |
pSL-PL118 |
SPslpA–ply118 fusion inserted into PstI/SalI digested pLC-PL118-P32, replacing ply118
|
This study |
pSL-PL511 |
SPslpA–ply511 fusion inserted into PstI/SalI digested pLC-PL118-P32, replacing ply118
|
This study |
pSL-PL511ΔC |
pSL-PL511 encoding C terminally truncated SPSlpA-Ply511Δ(S294–K373) |
This study |