FIGURE 1.

Sampling of an extinct chimney and microbial signal detections. (A) Bathymetric map of the southern Marina trough showing the Pika site and Guam. (B) Photograph showing the extinct chimney sampled by the remotely operated vehicle Hyper Dolphin. (C) Light microscopy image of a thin section from the extinct chimney. (D) Reflection light microscope image of the thin section. The inner chalcopyrite wall is indicated with arrows. (E) Fluorescence microscopy image of a grain boundary with greenish cell-like signals where a 3-μm thick focused ion beam (FIB) section was fabricated. (F) Ga ion image of a 3-μm thick FIB section from the grain boundary shown in (E). (G) Nanoscale secondary ion mass spectrometry (NanoSIMS) images of the FIB-fabricated grain boundary with intensity color contours. (H) Fluorescence microscopy image of a grain boundary with greenish cell-like signals where a 300-nm thick FIB section was fabricated. (I) Ga ion image of a 300-nm thick FIB section as pointed in (H). (J) NanoSIMS images of the FIB-fabricated grain boundary with intensity color contours.