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. 2022 Jun 7;13:911097. doi: 10.3389/fphys.2022.911097

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Copyright © 2022 Briggs, Hambly, Simão-Gurge, Garrison, Khaku, Van Susteren, Lewis, Riffell and Luckhart.

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Serotonergic and histaminergic innervation of A. stephensi tissues. (A) Schematic of the regions for immunohistochemistry. (B) Staining of the midgut for histamine (B ₁ ) and 5-HT (B ₂ ). Arrows denote the differences in staining for the lining of the midgut. (C) Staining of the thoracic ganglion for histamine (C ₁ ) and 5-HT (C ₂ ). Arrows denote blebby-like staining. (D) Staining of the brain for histamine (D ₁ ) and 5-HT (D ₂ ). Arrows denote stronger staining in the optic lobes for histamine and 5-HT.