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. 2022 Jun 7;16:901688. doi: 10.3389/fnins.2022.901688

FIGURE 5.

FIGURE 5

Upregulated p-Erk1/2 by extracellular ATP prevents autophagy induced by glutamate. Cultured SH-SY5Y cells were treated with or without ATP (5 and 10 μM), followed by the application of glutamate (10 mM) in the presence or absence of PD0325901 (50 nM) or rapamycin (50 nM) for 24 h; the untreated cells was the control group. (A) The expressions of LC3 and p62 were determined by western blot. Expression of GAPDH served as loading control. The quantitation of (B) p62 expression and (C) LC3 expression were calibrated. (D) The expressions of p-Erk1/2 and Erk1/2 were determined by western blot. Expression of GAPDH served as loading control. (E) The quantitation of p-Erk1/2 expression was calibrated. All data in bar charts present mean ± SEM from at least three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 versus the glutamate group or the ATP treated group. n.s., no significance.