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. 2022 Jun 7;13:771031. doi: 10.3389/fphar.2022.771031

FIGURE 2.

FIGURE 2

(A) Protein expressions of LC3I, LC3II, and α-SMA in NIH-3T3 cells treated with 5 ng/ml TGF-β1 with or without PIC (4 μM and 8 μM) for 24 h. (B) Quantitative Western blot analysis of LC3I/LC3II/α-SMA compared to GAPDH in different groups. (C) NIH-3T3 cells, transfected with the plasmid mRFP-GFP-LC3, were treated with PIC (4 μM) for 24 h in the existence or absence of stimulation by TGF-β (5 ng/ml). GFP and RFP were shown as LC3-positive cells and DAPI as nuclear. (D) Protein expressions of LC3I, LC3II, P62, Col1a1, α-SMA, and GAPDH in lung fibroblasts (NIH-3T3) treated with 5 ng/ml TGF-β1, 3 MA (2 mM) with or without PIC (4 μM) for 24 h. (E) Representative images of fibroblasts were treated with TGF-β1 and/or PIC (4 μM); then, autophagic vesicles (AVs) were analyzed by using a transmission electron microscope. The expression of protein was performed by ImageJ using GAPDH as a loading control. Data are expressed as mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.