Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Purification factor and activity recovery of malic enzyme from C. glutamicum during the different steps of protein purification
| Purification step | Total activity (μmol/min) | Yield (%) | Sp act (μmol · min−1 · mg of protein−1) | Purification factor |
|---|---|---|---|---|
| Crude extract | 47.4 | 100 | 0.124 | 1 |
| PEI | 46.5 | 98 | 0.16 | 1.3 |
| Sepharose Q | 30.3 | 64 | 2.6 | 21 |
| Blue trisacryl | 17 | 36 | 6.55 | 53 |
| Red Sepharose | 8 | 17 | 10.9 | 88 |
