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Effect of divalent metal ions, OAA, and glutamate on in vitro malic enzyme activity from C. glutamicuma
| Divalent cation | Concn (mM) | Activity (%) | Compound | Concn (mM) | Activity (%) |
|---|---|---|---|---|---|
| None | 12 | None | 100 | ||
| Mn2+ | 5 | 100 | OAA | 2.5 | 87 |
| 1 | 96 | 5 | 79 | ||
| Mg2+ | 5 | 94 | 10 | 67 | |
| 1 | 84 | ||||
| Co2+ | 5 | 56 | Glutamate | 25 | 92 |
| 1 | 73 | 100 | 88 | ||
| Ni2+ | 5 | 31 | 200 | 75 | |
| 1 | 37 | ||||
| Ca2+ | 5 | 5 | |||
| 1 | 0 |
All specific activities were measured at least three times per assay condition, and values are averaged values in which standard errors were less than ±5%.
