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. Author manuscript; available in PMC: 2022 Jul 10.
Published in final edited form as: Mol Psychiatry. 2022 Jan 10;27(3):1599–1610. doi: 10.1038/s41380-021-01390-w

Figure 3. SD increases 5-HT2AR levels in the PFC of WT mice in an Egr3-dependent manner.

Figure 3.

(A) 8h SD protocol. Quantification of 3H-M100907 binding autoradiography shows that SD, compared with SDc: (B) in AFC results in significantly greater 5-HT2AR levels in WT mice than Egr3−/− mice after SD (two-way ANOVA, sig. main effects of SD (F 1, 62 = 4.61, p = 0.0358) and genotype (F 1, 62 = 14.78, p = 0.0003); (C) in the PFC SD significantly upregulates 5-HT2AR levels in WT, but not Egr3−/−, mice (two-way ANOVA, sig. interaction between SD and genotype (F 1, 62 = 4.18, p = 0.0451)). (D) In the MPC, SD did not significantly increase 5-HT2AR levels; notably, 5-HT2ARs were lower in Egr3−/− mice than WT under both basal (SDc) and SD conditions (two-way ANOVA, sig. main effect of genotype (F 1, 62 = 38.79, p < 0.0001)) but not of SD (F 1, 62 = 1.371, p = 0.2461). Representative 3H-M100907 autoradiography images of brain tissue sections from (E) AFC, (F) PFC, and (G) MPC. For experiments in (B-D) WT: SDc, n = 17; SD, n = 16; Egr3−/−: SDc, n = 16; SD, n = 17. Bonferroni-corrected comparisons: * p < 0.05, ** p < 0.01, *** p < 0.001. Values represent means ± SEM.