Figure 3.

Enhancer regions upstream of pezo-1_{i and j} isoforms start codon drove GFP expression in the pharynx, and several sensory neurons. (A) 4.9-kb promoter spanning the largest intron in the pezo-1 gene was used to drive GFP expression of the shorter pezo-1 isoforms (using coelomocyte::GFP as co-injection marker). (B) GFP expression was present in pharyngeal muscles 3 (pm3), the marginal cells 1 (mc1) of the procorpus, and the anterior mechanosensitive neuron PVD. (C) Expression was seen in a pharyngeal neuron consistent with I3. (D) Expression was also detected in PHC, and the mechanosensitive neurons PVD (E). (F) An anterior ventral cell resembling the excretory gland was also labeled (*). (G) Unlike GFP expression driven by promoters targeting long isoform, fluorescence signals were evident in late embryos. (H) These expression patterns were confirmed by the PEZO-1::mScarlet (red) strain using magenta to tag the C-terminal end of all PEZO-1 proteins. All scale bars are 10 μm unless otherwise indicated. Animals are presented in sagittal views (dorsal up, anterior left). Observations reported based on N = 9 extrachromosomal (transcriptional reporter) and N = 6 integrated (translational reporter) animals. All images obtained using a Leica SP8 white light laser confocal microscope.