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. 2021 Dec 15;12(3):jkab429. doi: 10.1093/g3journal/jkab429

Figure 6.

Figure 6

Long pezo-1 isoforms are required for normal pharyngeal response to increases in food density. (A) Knockdown through RNAi (by feeding) of pezo-1a–h expression in a wild-type (N2) background resulted in an increase in pharyngeal pumping frequency (Mann–Whitney Rank Sum Test, N = 29). (B) Cultivation of WT (N2) C. elegans in bacteria lawns of increasing densities resulted in a decrease in pharyngeal pumping rate (red). However, mutations affecting long pezo-1 isoform (a–h) resulted in worms that displayed impaired responses to increased food density (N = 10). For statistical comparison, we grouped the data into Low (A600 = 0.32–0.34), Medium (A600 = 2.41–3.49), and High (A600 = 14.82–15.14) density. (C) Electropharyngeogram recordings of pezo-1 mutants induced to perform pharyngeal pumping by immersion in 5-mM serotonin solution also displayed pharyngeal pumping deficits including decreased pumping frequency, increased pump duration (D), and increased inter-pump intervals (E) (N = 10–26). For (C–E), One-way ANOVA on Ranks and Dunn’s on all Pairwise. Values reported are means ± SEMs. Please refer to Supplementary Table S4 for statistical comparisons between these groups.