Table 2.

Significant transcripts (P_FDR < 0.05) from TWAS of avenanthramides (AVNs) that have gene annotations where rank refers to overall transcript significance in TWAS analysis, and effect refers to the direction of correlation between expression and relative metabolite concentration. A list of all significant transcripts is provided in Supplementary Table 15.

Transcript id		AVN_A rank	AVN_A PFDR	AVN_B rank	AVN_B PFDR	Effect	Annotation
TRINITY_DN1008_c0_g2_i2	AB	15	0.002	19	0.004	Positive	Serine hydroxymethyltransferase 4
TRINITY_DN15878_c0_g1_i6	AB	17	0.002	34	0.008	Positive	Germacrene A hydroxylase
TRINITY_DN14541_c0_g1_i1	AB	18	0.002	31	0.008	Positive	Berberine bridge enzyme-like 18
TRINITY_DN26560_c0_g2_i1	AB	21	0.003	21	0.005	Positive	Phenylalanine ammonia-lyase
TRINITY_DN1103_c0_g1_i1	AB	22	0.003	32	0.008	Positive	Succinate-semialdehyde dehydrogenase, mitochondrial
TRINITY_DN2744_c0_g1_i4	AB	23	0.004	53	0.020	Positive	Fructose-bisphosphate aldolase 3, chloroplastic
TRINITY_DN29096_c0_g1_i9	AB	26	0.006	26	0.006	Positive	Probable purine permease 11
TRINITY_DN2577_c0_g1_i1	AB	31	0.008	33	0.008	Positive	Putative 12-oxophytodienoate reductase 11
TRINITY_DN3411_c0_g1_i4	AB	38	0.015	57	0.024	Positive	Transketolase, chloroplastic
TRINITY_DN16295_c0_g1_i1	AB	41	0.015	30	0.007	Positive	Mixed-linked glucan synthase 2
TRINITY_DN3916_c0_g1_i1	AB	47	0.023	36	0.010	Positive	ALA-interacting subunit 1
TRINITY_DN1581_c0_g1_i3	AB	48	0.023	22	0.005	Positive	Phosphoenolpyruvate/phosphate translocator 1, chloroplastic
TRINITY_DN784_c0_g1_i3	AB	55	0.027	13	0.002	Positive	Probable methylenetetrahydrofolate reductase
TRINITY_DN2924_c0_g1_i2	AB	66	0.039	15	0.004	Positive	Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform
TRINITY_DN13684_c0_g1_i1	AB	70	0.047	56	0.023	Positive	Aconitate hydratase 3, mitochondrial
TRINITY_DN512_c0_g2_i1	A	30	0.008	160	0.171	Positive	Phosphoenolpyruvate carboxylase 2
TRINITY_DN13998_c0_g1_i1	A	39	0.015	122	0.123	Positive	Xylanase inhibitor protein 1
TRINITY_DN1272_c0_g1_i3	A	45	0.021	217	0.218	Positive	Sucrose transport protein SUT1; N
TRINITY_DN3267_c0_g1_i1	A	53	0.027	NS	NS	Negative	Pentatricopeptide repeat-containing protein At2g15690, mitochondrial
TRINITY_DN14356_c1_g1_i10	A	54	0.027	151	0.169	Positive	Isoflavone 2'-hydroxylase
TRINITY_DN11233_c0_g1_i7	A	58	0.030	260	0.231	Positive	Cytochrome P450 81D11
TRINITY_DN20857_c0_g1_i4	A	62	0.035	99	0.090	Positive	S-adenosylmethionine decarboxylase proenzyme
TRINITY_DN9961_c0_g1_i7	A	71	0.047	74	0.053	Positive	Endo-1,4-beta-xylanase5
TRINITY_DN7337_c0_g3_i1	A	72	0.050	172	0.180	Positive	Probable metal-nicotianamine transporter YSL12
TRINITY_DN19061_c0_g1_i1	B	86	0.073	38	0.010	Positive	Aldehyde dehydrogenase family 2 member C4
TRINITY_DN2385_c0_g1_i1	B	115	0.103	61	0.031	Positive	Transketolase, chloroplastic
TRINITY_DN2667_c0_g1_i1	B	155	0.150	66	0.041	Positive	Probable nitronate monooxygenase
TRINITY_DN1363_c0_g1_i2	B	163	0.169	67	0.044	Negative	Serine/threonine-protein kinase rio2
TRINITY_DN4266_c0_g1_i6	B	211	0.233	43	0.013	Positive	Probable inositol oxygenase
TRINITY_DN28530_c0_g1_i4	B	NS	NS	35	0.008	Positive	Threonine synthase 1, chloroplastic
TRINITY_DN2212_c0_g1_i2	B	NS	NS	68	0.044	Negative	Eukaryotic translation initiation factor 2 subunit 3

A full list of all significant transcripts is in Supplementary Table S15. Rank refers to overall transcript significance in TWAS analysis, and effect refers to the direction of correlation between expression and relative concentration of avenanthramide.