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Geranic acid formation by cell-free cytosolic extractsa
| Extract type | Protein content (mg ml−1) | Myrcene provided (mM) | Geranic acid formed (mM) |
|---|---|---|---|
| Complete | 10.1 | 10 | 0.590 |
| No myrcene | 10.1 | 0 | 0.000 |
| With heat treatment (95°C for 10 min) | 10.1 | 10 | 0.000 |
| With nitrate (20 mM) | 10.1 | 10 | 0.581 |
| Diluted (1:5) | 1.85 | 10 | 0.127 |
| Diluted (1:10) | 1.15 | 10 | 0.029 |
Assays were incubated for 20 h.
