Figure 2.

Whole ovary RNA-seq dataset overview. (A) hierarchical clustering dendrogram and (B) PCA of the whole ovary RNA-seq dataset, both showing that biological replicates are similar to each other, and that early and mid-stages are more similar to each other than either of them is to late stage. (C) Number of differentially expressed genes between early and mid stages, and between mid and late stages (adjusted P-value < 0.01; black: upregulated genes; white: downregulated genes). See Supplementary Table S2 for gene list. (D) Number of significantly upregulated stage-specific genes (adjusted P-value < 0.01). See Supplementary Table S3 for gene list. (E) Heatmap showing the expression of all the stage-specific upregulated genes as a row-wise z-score. Genes are clustered hierarchically and separated into three groups using the function “cutree,” and grayscale row labels (“*Gene DE”) immediately to the right of the tree are colored based on the stage in which the gene was detected to be significantly upregulated (x-axis categories).