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. 2021 Dec 21;12(3):jkab422. doi: 10.1093/g3journal/jkab422

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© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Isoform Classification (A) SQANTI3 isoform classification explanation, FSM, full-splice match; ISM, incomplete-splice match; NIC, novel in catalog; NNC, novel not in catalog. Genic Genomic = overlaps introns and exons. For (B) through (E) Left: PacBio-only. Right: PacBio+Reference Merged dataset. (B) Length distribution of transcripts, shaded by classification. (C) Isoforms per gene. (D) Percentage of predicted NMD by classification. (E) Example of the improved transcriptome by combining existing reference (top, gray) with the PacBio Iso-Seq transcriptome (middle, pink) into a merged (bottom, purple) transcriptome (see also Supplementary Figure S3).