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. 2022 May 24;4(1):vdac079. doi: 10.1093/noajnl/vdac079

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© The Author(s) 2022. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Histopathological validation of allograft DMG mouse models. (A) Representative coronal sections (top panels) and high magnification (400×, bottom panels) images of H&E stained DMG allograft tumors across genetic conditions. (B) Representative images (400×) of Ki67 immunohistochemical staining (in brown) of DMG allograft tumor sections showing the tumor core (top panels) and diffuse, infiltrative growth areas (bottom panels). (C) Representative images (400×) of immunohistochemical staining (in brown) for nuclear mutant histone 3 protein (H3K27M, top panels) and histone 3 lysine 27 di- and trimethylation (H3K27me2/3, bottom panels) of DMG allograft tumor sections. Scale bars = 50 μm.