Table 1.
Azole-susceptibility of environmental A. fumigatus isolates from Georgia and Florida, USA with nonsynonymous cyp51A mutations
| Isolates | cyp51A genotypea | MIC Ranges (μg/ml)b |
|||
|---|---|---|---|---|---|
| TEB | ITC | VOR | POS | ||
| A1163 | WT | 1.0 | 1.0 | 0.25 | 0.25 |
| eAF221 | TR46/Y121F/T289A | >16 | 1.0-2.0 | >16 | 1.0 |
| eAF222 | TR46/Y121F/T289A | >16 | 2.0 | >16 | 1.0 |
| eAF228 | TR46/Y121F/T289A | >16 | 1.0-2.0 | >16 | 1.0 |
| eAF229 | TR46/Y121F/T289A | >16 | 1.0-2.0 | >16 | 1.0 |
| eAF230 | TR46/Y121F/T289A | >16 | 1.0-2.0 | >16 | 1.0 |
| eAF231 | TR46/Y121F/T289A | >16 | 1.0-2.0 | >16 | 1.0 |
| eAF232 | TR46/Y121F/T289A | >16 | 1.0-2.0 | >16 | 1.0 |
| eAF233 | TR46/Y121F/T289A | >16 | 1.0-2.0 | >16 | 1.0 |
| eAF234 | TR46/Y121F/T289A | >16 | 1.0-2.0 | >16 | 1.0 |
| eAF235 | TR46/Y121F/T289A | 16 | 1.0 | >16 | 0.5-1.0 |
| eAF236 | TR46/Y121F/T289A | >16 | 1.0-2.0 | >16 | 1.0 |
| eAF513 | TR46/Y121F/T289A | >16 | 1.0 | >16 | 1.0 |
| eAF010 | I242V | 1.0-2.0 | 1.0 | 0.5 | 0.5-1.0 |
| eAF175 | I242V | 2.0 | 1.0 | 0.25-0.5 | 0.5 |
| eAF263 | I242V | 2.0 | 1.0 | 0.5 | 0.5 |
| eAF265 | I242V | 2.0 | 1.0 | 0.5 | 0.5 |
| eAF406 | I242V | 2.0 | 1.0 | 0.5 | 0.25 |
| eAF500 | I242V | 2.0 | 1.0 | 0.5 | 0.5 |
| eAF589 | I242V | 2.0 | 1.0 | 0.25-0.5 | 0.25-0.5 |
| eAF621 | I242V | 2.0 | 1.0 | 0.5 | 0.5 |
| eAF647 | I242V | 2.0 | 1.0 | 0.25 | 0.25 |
| eAF773 | I242V | 2.0 | 1.0 | 0.5 | 0.5 |
| eAF792 | I242V | 1.0-2.0 | 0.5-1.0 | 0.25-0.5 | 0.25-0.5 |
| eAF016 | Y46F/V172M/T248N/E255D/K427E | 1.0-2.0 | 1.0 | 0.25-0.5 | 0.5 |
| eAF082 | Y46F/V172M/T248N/E255D/K427E | 1.0-2.0 | 1.0 | 0.25-0.5 | 0.25-0.5 |
| eAF128 | Y46F/V172M/T248N/E255D/K427E | 2.0 | 1.0 | 0.5 | 0.5 |
| eAF163 | Y46F/V172M/T248N/E255D/K427E | 1.0-2.0 | 1.0 | 0.25-0.5 | 0.5 |
| eAF609 | Y46F/V172M/T248N/E255D/K427E | 2.0-4.0 | 1.0 | 0.5-1.0 | 0.25-0.5 |
a
Cyp51A protein GenBank accession number EDP50065.1 used as reference.
b
MICs, Minimal Inhibitory Concentrations; TEB, tebuconazole; ITC, itraconazole; VOR, voriconazole; POS, posaconazole. Each assay was conducted twice. A single value is listed if the result was the same for both assays and a range was listed if the results differed between assays.