Table 2.
Mutations associated with fungicide resistance in pan-azole-resistant A. fumigatus
Isolate | Source | cyp51A—azolesa | cytB—QoIb | benA—MBCc |
---|---|---|---|---|
A1163 | Clinic | WT | WT | WT |
eAF222 | Environment, 2018 USA | TR46/Y121F/T289A | G143A | F219Y |
eAF233 | Environment, 2018 USA | TR46/Y121F/T289A | G143A | F219Y |
eAF234 | Environment, 2018 USA | TR46/Y121F/T289A | G143A | F219Y |
eAF513 | Environment, 2018 USA | TR46/Y121F/T289A | G143A | F219Y |
08-12-12-13 | Clinic, 2003 Netherlands | TR34/L98H/ | WT | F219Y |
08-31-08-91 | Clinic, 2004 Netherlands | TR34/L98H | WT | F219Y |
08-36-03-25 | Clinic, 2005 Netherlands | TR34/L98H | WT | F219Y |
10-01-02-27 | Clinic, 2010 Netherlands | TR34/L98H | G143A | F219Y |
Afu 942/09 | Clinic, 2009 India | TR34/L98H | G143A | F219Y |
Afu 1042/09 | Clinic, 2009 India | TR34/L98H | G143A | F219Y |
Afu 124/E11 | Environment, 2011 India | TR34/L98H | G143A | F219Y |
Afu 166/E11 | Environment, 2011 India | TR34/L98H | G143A | F219Y |
Afu 218/E11 | Environment, 2011 India | TR34/L98H | G143A | F219Y |
Afu 257/E11 | Environment, 2011 India | TR34/L98H | G143A | F219Y |
Afu 343/P11 | Clinic, 2011 India | TR34/L98H | G143A | F219Y |
Afu 591/12 | Clinic, 2012 India | TR34/L98H | G143A | F219Y |
DI 15-96 | Clinic, 2008 USA | TR46/Y121F/T289A | G143A | F219Y |
DI 15-102 | Clinic, 2010 USA | TR34/L98H | WT | F219Y |
DI 15-106 | Clinic, 2012 USA | TR46/Y121F/T289A | G143A | F219Y |
DI 15-116 | Clinic, 2014 USA | TR34/L98H | WT | F219Y |
GenBank accession number EDP50065 from azole-sensitive isolate A1163 was used as wildtype for Cyp51A. Isolates 08-12-12-13 and 08-36-03-25 also carried S297T/F495I mutations for Cyp51A, but these have not been associated with azole resistance.
GenBank accession number YP_005353050 from azole-sensitive isolate A1163 was used as wildtype for CytB. All cyp51A TR mutants also carried V13I/I119V mutations for CytB, but these have not been associated with QoI resistance.
GenBank accession number EDP56324 from azole-sensitive isolate A1163 was used as wildtype for BenA.