Figure 3.

Embryonic and monocyte-derived adrenal gland macrophages are distinct subsets identified through CX3CR1 expression

(A) (Left) Representative plot of macrophage CX3CR1-GFP and R26^TdTomato expression and (right) proportions of CX3CR1-GFP⁺ and CX3CR1-GFP^- cells among R26^TdTomato+ AGMs from (n = 3) female CX3CR1^GFP/+ CCR2^CreERT2/+ R26^TdTomato mice 48 h post TAM gavage. Data are from one experiment.

(B) Representative plot of macrophage CX3CR1 and R26^TdTomato expression in double reporter CX3CR1^creERT2/GFP R26^TdTomato mice 48 h post TAM administration. Data are from one experiment.

(C) Representative plot of macrophage CX3CR1 and R26^TdTomato expression in double reporter CX3CR1^creERT2/GFP R26^TdTomato mice 7 days post tamoxifen administration. Data are from one experiment.

(D) Flow-cytometry plot showing Timd4 and CX3CR1 expression by AGMs. Data are representative of at least 4 independent experiments.

(E) Embryonic labeling of CX3CR1^creERT2 R26^TdTomato mice was performed at E14.5. R26^TdTomato+ cells were identified in 8-week-old male (n = 4) and female (n = 2) offspring. Data are representative of 2 independent experiments.

(F) Embryonic labeling of CX3CR1^creERT2 R26^TdTomato mice was performed at E18.5. R26^TdTomato+ cells were identified in 10-week-old female offspring. Scale bar: 200 μm. Data are representative of 2 independent experiments.

(G) Flow-cytometry analysis of Timd4 and MHC class II expression in AGMs from E18–E20 embryos and male adult (9-week-old) mice. Data are from one experiment.

(H) Embryonic labeling of CX3CR1^creERT2 R26^TdTomato mice was performed at E14.5. R26^TdTomato+ cells were identified in 1-week-old male offspring (left panel), which comprised mainly Tidm4⁺ Lyve1⁺ cells (right panel). Data are representative of n = 2 mice. Data are from one experiment. Two-tailed Mann-Whitney tests were used for statistical analysis.

See also Figure S3.