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. 2022 Jun 14;39(11):110949. doi: 10.1016/j.celrep.2022.110949

Figure 5.

Figure 5

Adrenal gland macrophage sex-dimorphism is established with organ maturation and depends on X-zone presence

(A) Representative plots showing AGM MHC-II and Lyve1 expression in male 1- or 7-week-old mice. Data representative of 2 independent experiments.

(B) Proportions of MHC-IIlow Lyve1+ macrophages in male and female 1-(♂ n = 8, ♀ n = 8), 3-(♂ n = 3, ♀ n = 3) or 7 to 9-week-old (♂ n = 13, ♀ n = 13) mice. Data pooled from 2 independent experiments.

(C) Quantification of MHC-IIhigh AGMs in male and female 1- (♂ n = 9, ♀ n = 7) or 7-week-old (♂ n = 5, ♀ n = 5) mice. Data are pooled from 2 independent experiments.

(D) (Left) Representative plots showing MHC class II expression and (right) proportions of MHC class IIlow macrophages in male and female 4- (♂ n = 4, ♀ n = 3) and 5-week-old (♂ n = 7, ♀ n = 10) wild-type mice. Data are pooled from 2 independent experiments.

(E) (Left) Representative plots showing MHC class II expression and (right) proportions of MHC class IIlow AGMs in 7-week-old castrated (n = 6) and sham-operated (n = 6) wild-type male mice. Data are pooled from 2 independent experiments.

(F) Confocal-microscopy analysis of MHC class II and CD68 expression in 7-week-old castrated and sham-operated wild-type mice. The X-zone is comprised between white and orange dots. Scale bar: 200 μm. M, medulla; C, cortex; ZX, X- zone. Data are representative of 2 independent experiments.

(G) (Left) Representative plots showing MHC class II expression and (right) proportions of MHC class IIlow AGMs in female retired breeders (n = 7) and age-matched nulliparous (n = 6) mice. Data are pooled from 2 independent experiments.

(H) Fluorescence-microscopy analysis of CD68 and MHC class II expression in adrenal glands from 12-week-old female retired breeders and age-matched nulliparous mice. The X zone is comprised between white and orange dots. Scale bar: 100 μm. M, medulla; C, cortex. Data are representative of 2 independent experiments.

(I) Proportions of MHC class IIlow macrophages in female mice treated with anti-IL-10R-blocking antibody or isotype control. Data are pooled from 3 independent experiments.

Statistical analysis was performed using two-way ANOVA with Bonferroni’s post-test (B–D) or two-tailed Mann-Whitney tests (E, G, and I).

See also Figure S5.