Figure 6.

Adrenal gland macrophages control tissue lipid metabolism

(A) (Left) Representative plots and (right) quantification of AGMs in 7- to 8-week-old CX3CR1^GFP/+ mice after macrophage depletion using α-CD115 (n = 5) or isotype control (n = 5).

(B) Microscopy analysis of adrenal glands from α-CD115- or isotype-control-treated CX3CR1^GFP/+ mice. One microscopy experiment was performed to confirm depletion efficiency.

(C) Microscopy analysis of adrenal glands from α-CD115- or isotype-control-treated male mice using Bodipy staining. Data are from one experiment.

(D) Quantification of Bodipy⁺ particles of different sizes in adrenal glands from α-CD115- (n = 3) or isotype-control- (n = 3) treated male mice. Data are from one experiment.

(E) Aldosterone and corticosterone levels in adrenal gland homogenates from 8-week-old α-CD115- (n = 5) or isotype-control- (n = 5) treated female mice submitted to a 12 h cold challenge. Data are from one experiment.

(F) Quantification of Bodipy⁺ particles of different sizes in adrenal glands from 8-week-old α-CD115- (n = 5) or isotype-control- (n = 5) treated female mice submitted to a 12 h cold challenge.

Statistical analysis was performed using two-way ANOVA with Bonferroni’s post-test (D and F) or two-tailed Mann-Whitney tests (A and E).

See also Figure S6.