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. 2022 Jun 14;39(11):110949. doi: 10.1016/j.celrep.2022.110949
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

CD11b PE-Cy5 (Clone M1/70) Biolegend Cat# 101209, RRID:AB_312792
CD11b Brilliant Violet 510 (Clone M1/70) Biolegend Cat# 101263, RRID:AB_2629529
CD11b APC-Cy7 (Clone M1/70) Biolegend Cat# 101226, RRID:AB_830642
F4/80 PE-Cy7 (Clone BM8) Biolegend Cat# 123114, RRID:AB_893478
F4/80 Alexa Fluor 488 (Clone BM8) Biolegend Cat# 123120, RRID:AB_893479
F4/80 Brilliant Violet 650 (Clone BM8) Biolegend Cat# 123149, RRID:AB_2564589
F4/80 APC (Clone REA126) Miltenyi Cat# 130-116-525 RRID:AB_2733417
CD45 APC-Cy7 (Clone 30-F11) BD Biosciences Cat# 557659, RRID:AB_396774
CD45 Pacific Blue (Clone 30-F11) Biolegend Cat# 103126, RRID:AB_493535
CD45 VioGreen (Clone REA737) Miltenyi Cat# 130-110-803, RRID: AB_2658224
CD45 Brilliant Violet 570 (Clone 30-F11) Biolegend Cat# 103136, RRID:AB_10898325
CD45 APC-Vio 770 (Clone REA737) Miltenyi Cat# 130-110-662, RRID:AB_2658231
CD64 Brillant Violet 421 (Clone ×54-5/7.1) Biolegend Cat# 139309, RRID: AB_2562694
CD64 Brillant Violet 711 (Clone ×54-5/7.1) Biolegend Cat# 139311, RRID: AB_2563846
CD64 PE/Dazzle 594 (Clone ×54-5/7.1) Biolegend Cat# 139320, RRID:AB_2566559
Ly6C APC (Clone HK1.4) Biolegend Cat# 128015, RRID:AB_1732087
Ly6C PerCP-Cy5.5 (Clone HK1.4) Biolegend Cat# 128012, RRID: AB_1659241
Ly6G PerCP-Cy5.5 (Clone 1A8) Biolegend Cat# 127615, RRID:AB_1877272
Ly6G Brilliant Violet 510 (Clone 1A8) Biolegend Cat#127633, RRID: AB_2562937
Ly6G Brilliant Violet 785 (Clone 1A8) Biolegend Cat# 127645, RRID:AB_2566317
Klrg1 PE-Cy7 (Clone 2F1/KLRG1) Biolegend Cat# 138416, RRID:AB_2561736
CD115 PE-Cy7 (AFS98) Biolegend Cat# 135524, RRID: AB_2566460
CD115 PE (Clone AFS98) Biolegend Cat# 135506, RRID:AB_1937253
CD115 PE (Clone REA827) Miltenyi Cat# 130-112-639, RRID:AB_2654553
Gr-1 PerCP-Cy5.5 (Clone RB6-8C5) Biolegend Cat# 108426, RRID:AB_893557
NK1.1 FITC (Clone PK136) Biolegend Cat# 108706, RRID:AB_313393
Sca1 Pacific Blue (Clone D7) Biolegend Cat# 108120, RRID:AB_493273
CD19 PE (Clone REA749) Miltenyi Cat# 130-112-035, RRID:AB_2655822
CD19 BUV737 (Clone 1D3) BD Biosciences Cat# 612782, RRID:AB_2870111
CD3 APC (Clone 17A2) Biolegend Cat# 100236, RRID:AB_2561456
CD8a Brilliant Violet 510 (Clone 53-6.7) Biolegend Cat# 100752, RRID:AB_2563057
CD4 Alexa Fluor 700 (Clone RM4-5) Biolegend Cat# 100536, RRID:AB_493701
MHC-II IA/IE PE (Clone 2G9) BD Biosciences Cat# 558593, RRID:AB_647221
MHC-II IA/IE FITC (Clone 2G9) BD Biosciences Cat# 553623, RRID:AB_394958
MHC-II IA/IE Brilliant Violet 510 (Clone M5/114.15.2) Biolegend Cat# 107636, RRID : AB_2734168
MHC-II IA/IE Alexa Fluor 647 (Clone M5/114.15.2) Biolegend Cat# 107618, RRID:AB_493525
MHC-II VioBlue (Clone REA813) Miltenyi Cat# 130-112-394, RRID:AB_2652908
Timd4 PE-Cy7 (Clone RMT4-54) Biolegend Cat# 130010, RRID:AB_2565719
Timd4 PerCP-eFluor710 (Clone RMT4-54) Invitrogen Cat# 46-5866-80, RRID:AB_2573780
Lyve1 eFluor660 (Clone ALY7) Invitrogen Cat# 50-0443-80, RRID:AB_10598060
CD206 PerCP-Cy5.5 (Clone C068C2) Biolegend Cat# 141716, RRID:AB_2561992
MerTK PE (Clone 2B10C42) Biolegend Cat# 151506, RRID:AB_2617037
MerTK APC (Clone 2B10C42) Biolegend Cat# 151508, RRID: AB_2650739
CD11c PE-Cy7 (Clone HL3) BD Biosciences Cat# 558079, RRID:AB_647251
CD24 BUV496 (Clone M1/69) BD Biosciences Cat# 612953, RRID:AB_2870229
NKp46 Brilliant Violet 421 (Clone 29A1.4) Biolegend Cat# 137611, RRID:AB_10915472
CCR2 APC-Fire750 (Clone SA203T11) Biolegend Cat# 150629, RRID :AB_2810416
CD40 BUV395 (Clone 3/23) BD Biosciences Cat# 745697, RRID: AB_2743179
CD40 APC-Vio770 (Clone REA965) Miltenyi Cat# 130-116-113, RRID:AB_2727355
CD80 BUV615 (Clone 16-10A1) BD Biosciences Cat# 751328, RRID:AB_2875337
CD80 PerCP-Vio770 (Clone REA983) Miltenyi Cat# 130-116-464, RRID:AB_2727561
CD86 BUV805 (Clone GL1) BD Biosciences Cat# 741946, RRID:AB_2871258
CD86 PE (Clone REA1190) Miltenyi Cat# 130-122-129, RRID:AB_2819412
ICOSL (CD275) PE-Vio770 (Clone REA990) Miltenyi Cat# 130-116-448, RRID:AB_2727549
CD68 Alexa Fluor 647 (Clone FA-11) Biolegend Cat# 137004, RRID:AB_2044002
InVivoMAb anti-mouse CSF1R (Clone AFS98) BioXCell Cat# BE0213, RRID:AB_2687699
InVivoMAb rat IgG2a isotype control, anti-trinitrophenol (Clone 2A3) BioXCell Cat# BE0089, RRID:AB_1107769
InVivoMAb anti-mouse IL10R (Clone BE0050) BioXCell Cat# BE0050, RRID:AB_1107611
InVivoMAb anti-mouse CD16/CD32 (Clone 2.4G2) BioXCell Cat# BE0307, RRID:AB_2736987

Chemicals, peptides, and recombinant Proteins

DAPI Sigma Cat# D9542
LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit ThermoFisher Cat# L34955
PFA 4% VWR International Cat# 9713.1000
Bovine serum Albumin (BSA) Sigma Cat# A7030
Tamoxifen Sigma Cat# T5648
Collagenase A Sigma Cat# 11088793001
IHC Antigen retrieval solution eBiosciences Cat# 00-4955-58
Antifade mounting medium with DAPI Vectashield Cat# H-1500
Fetal bovine serum Fisher Scientific Cat# 12350273
Lysing buffer BD Biosciences Cat# 555899
Liberase Roche Cat# 05401054001
DNAse I Roche Cat# 10104159001
Penicillin Streptomycin Life Technologies Cat# 15070063
L-Glutamine Life Technologies Cat# 25030024
Mouse M-CSF Miltenyi Biotec Cat# 130-094-129
RPMI medium Life Technologies Cat# 21875091
Clorgyline Abcam Cat# ab145646
Norepinephrine Sigma Cat# A7257
TRITC-Dextran 65–85 kDa Sigma Cat# T1162
Bodipy Thermofisher Cat# D3922

Critical commercial assays

Mouse CCL2 DuoSet ELISA R&D Systems Cat# DY479-05
Norepinephrine ELISA Kit Tebu-bio Cat# 157KA1891
Aldosterone Parameter Assay Kit R&D Systems Cat# KGE016
Corticosterone Parameter Assay Kit R&D Systems Cat# KGE009
Ki67 Staining Kit PE BD Biosciences Cat# 51-36525×
FoxP3 Staining Buffer Set Miltenyi Cat# 130-093-142
RNA extraction kit QIAGEN Cat# 74136

Software and algorithms

Prism 8 GraphPad N/A
Chromeleon software Thermo Scientific N/A
FlowJo Tree Star N/A
Seurat package version 3.1.0 https://satijalab.org/seurat/ N/A
BD FACSDiva BD Biosciences N/A
ImageJ NIH N/A
SpectroFlo Cytek N/A
Phantasus Artyomov Lab N/A