Antibodies |
|
CD11b PE-Cy5 (Clone M1/70) |
Biolegend |
Cat# 101209, RRID:AB_312792
|
CD11b Brilliant Violet 510 (Clone M1/70) |
Biolegend |
Cat# 101263, RRID:AB_2629529
|
CD11b APC-Cy7 (Clone M1/70) |
Biolegend |
Cat# 101226, RRID:AB_830642
|
F4/80 PE-Cy7 (Clone BM8) |
Biolegend |
Cat# 123114, RRID:AB_893478
|
F4/80 Alexa Fluor 488 (Clone BM8) |
Biolegend |
Cat# 123120, RRID:AB_893479
|
F4/80 Brilliant Violet 650 (Clone BM8) |
Biolegend |
Cat# 123149, RRID:AB_2564589
|
F4/80 APC (Clone REA126) |
Miltenyi |
Cat# 130-116-525 RRID:AB_2733417
|
CD45 APC-Cy7 (Clone 30-F11) |
BD Biosciences |
Cat# 557659, RRID:AB_396774
|
CD45 Pacific Blue (Clone 30-F11) |
Biolegend |
Cat# 103126, RRID:AB_493535
|
CD45 VioGreen (Clone REA737) |
Miltenyi |
Cat# 130-110-803, RRID: AB_2658224
|
CD45 Brilliant Violet 570 (Clone 30-F11) |
Biolegend |
Cat# 103136, RRID:AB_10898325
|
CD45 APC-Vio 770 (Clone REA737) |
Miltenyi |
Cat# 130-110-662, RRID:AB_2658231
|
CD64 Brillant Violet 421 (Clone ×54-5/7.1) |
Biolegend |
Cat# 139309, RRID: AB_2562694
|
CD64 Brillant Violet 711 (Clone ×54-5/7.1) |
Biolegend |
Cat# 139311, RRID: AB_2563846
|
CD64 PE/Dazzle 594 (Clone ×54-5/7.1) |
Biolegend |
Cat# 139320, RRID:AB_2566559
|
Ly6C APC (Clone HK1.4) |
Biolegend |
Cat# 128015, RRID:AB_1732087
|
Ly6C PerCP-Cy5.5 (Clone HK1.4) |
Biolegend |
Cat# 128012, RRID: AB_1659241
|
Ly6G PerCP-Cy5.5 (Clone 1A8) |
Biolegend |
Cat# 127615, RRID:AB_1877272
|
Ly6G Brilliant Violet 510 (Clone 1A8) |
Biolegend |
Cat#127633, RRID: AB_2562937
|
Ly6G Brilliant Violet 785 (Clone 1A8) |
Biolegend |
Cat# 127645, RRID:AB_2566317
|
Klrg1 PE-Cy7 (Clone 2F1/KLRG1) |
Biolegend |
Cat# 138416, RRID:AB_2561736
|
CD115 PE-Cy7 (AFS98) |
Biolegend |
Cat# 135524, RRID: AB_2566460
|
CD115 PE (Clone AFS98) |
Biolegend |
Cat# 135506, RRID:AB_1937253
|
CD115 PE (Clone REA827) |
Miltenyi |
Cat# 130-112-639, RRID:AB_2654553
|
Gr-1 PerCP-Cy5.5 (Clone RB6-8C5) |
Biolegend |
Cat# 108426, RRID:AB_893557
|
NK1.1 FITC (Clone PK136) |
Biolegend |
Cat# 108706, RRID:AB_313393
|
Sca1 Pacific Blue (Clone D7) |
Biolegend |
Cat# 108120, RRID:AB_493273
|
CD19 PE (Clone REA749) |
Miltenyi |
Cat# 130-112-035, RRID:AB_2655822
|
CD19 BUV737 (Clone 1D3) |
BD Biosciences |
Cat# 612782, RRID:AB_2870111
|
CD3 APC (Clone 17A2) |
Biolegend |
Cat# 100236, RRID:AB_2561456
|
CD8a Brilliant Violet 510 (Clone 53-6.7) |
Biolegend |
Cat# 100752, RRID:AB_2563057
|
CD4 Alexa Fluor 700 (Clone RM4-5) |
Biolegend |
Cat# 100536, RRID:AB_493701
|
MHC-II IA/IE PE (Clone 2G9) |
BD Biosciences |
Cat# 558593, RRID:AB_647221
|
MHC-II IA/IE FITC (Clone 2G9) |
BD Biosciences |
Cat# 553623, RRID:AB_394958
|
MHC-II IA/IE Brilliant Violet 510 (Clone M5/114.15.2) |
Biolegend |
Cat# 107636, RRID : AB_2734168 |
MHC-II IA/IE Alexa Fluor 647 (Clone M5/114.15.2) |
Biolegend |
Cat# 107618, RRID:AB_493525
|
MHC-II VioBlue (Clone REA813) |
Miltenyi |
Cat# 130-112-394, RRID:AB_2652908
|
Timd4 PE-Cy7 (Clone RMT4-54) |
Biolegend |
Cat# 130010, RRID:AB_2565719
|
Timd4 PerCP-eFluor710 (Clone RMT4-54) |
Invitrogen |
Cat# 46-5866-80, RRID:AB_2573780
|
Lyve1 eFluor660 (Clone ALY7) |
Invitrogen |
Cat# 50-0443-80, RRID:AB_10598060
|
CD206 PerCP-Cy5.5 (Clone C068C2) |
Biolegend |
Cat# 141716, RRID:AB_2561992
|
MerTK PE (Clone 2B10C42) |
Biolegend |
Cat# 151506, RRID:AB_2617037
|
MerTK APC (Clone 2B10C42) |
Biolegend |
Cat# 151508, RRID: AB_2650739
|
CD11c PE-Cy7 (Clone HL3) |
BD Biosciences |
Cat# 558079, RRID:AB_647251
|
CD24 BUV496 (Clone M1/69) |
BD Biosciences |
Cat# 612953, RRID:AB_2870229
|
NKp46 Brilliant Violet 421 (Clone 29A1.4) |
Biolegend |
Cat# 137611, RRID:AB_10915472
|
CCR2 APC-Fire750 (Clone SA203T11) |
Biolegend |
Cat# 150629, RRID :AB_2810416 |
CD40 BUV395 (Clone 3/23) |
BD Biosciences |
Cat# 745697, RRID: AB_2743179
|
CD40 APC-Vio770 (Clone REA965) |
Miltenyi |
Cat# 130-116-113, RRID:AB_2727355
|
CD80 BUV615 (Clone 16-10A1) |
BD Biosciences |
Cat# 751328, RRID:AB_2875337
|
CD80 PerCP-Vio770 (Clone REA983) |
Miltenyi |
Cat# 130-116-464, RRID:AB_2727561
|
CD86 BUV805 (Clone GL1) |
BD Biosciences |
Cat# 741946, RRID:AB_2871258
|
CD86 PE (Clone REA1190) |
Miltenyi |
Cat# 130-122-129, RRID:AB_2819412
|
ICOSL (CD275) PE-Vio770 (Clone REA990) |
Miltenyi |
Cat# 130-116-448, RRID:AB_2727549
|
CD68 Alexa Fluor 647 (Clone FA-11) |
Biolegend |
Cat# 137004, RRID:AB_2044002
|
InVivoMAb anti-mouse CSF1R (Clone AFS98) |
BioXCell |
Cat# BE0213, RRID:AB_2687699
|
InVivoMAb rat IgG2a isotype control, anti-trinitrophenol (Clone 2A3) |
BioXCell |
Cat# BE0089, RRID:AB_1107769
|
InVivoMAb anti-mouse IL10R (Clone BE0050) |
BioXCell |
Cat# BE0050, RRID:AB_1107611
|
InVivoMAb anti-mouse CD16/CD32 (Clone 2.4G2) |
BioXCell |
Cat# BE0307, RRID:AB_2736987
|
|
Chemicals, peptides, and recombinant Proteins |
|
DAPI |
Sigma |
Cat# D9542 |
LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit |
ThermoFisher |
Cat# L34955
|
PFA 4% |
VWR International |
Cat# 9713.1000 |
Bovine serum Albumin (BSA) |
Sigma |
Cat# A7030 |
Tamoxifen |
Sigma |
Cat# T5648 |
Collagenase A |
Sigma |
Cat# 11088793001 |
IHC Antigen retrieval solution |
eBiosciences |
Cat# 00-4955-58 |
Antifade mounting medium with DAPI |
Vectashield |
Cat# H-1500 |
Fetal bovine serum |
Fisher Scientific |
Cat# 12350273 |
Lysing buffer |
BD Biosciences |
Cat# 555899 |
Liberase |
Roche |
Cat# 05401054001 |
DNAse I |
Roche |
Cat# 10104159001 |
Penicillin Streptomycin |
Life Technologies |
Cat# 15070063 |
L-Glutamine |
Life Technologies |
Cat# 25030024 |
Mouse M-CSF |
Miltenyi Biotec |
Cat# 130-094-129 |
RPMI medium |
Life Technologies |
Cat# 21875091 |
Clorgyline |
Abcam |
Cat# ab145646 |
Norepinephrine |
Sigma |
Cat# A7257 |
TRITC-Dextran 65–85 kDa |
Sigma |
Cat# T1162 |
Bodipy |
Thermofisher |
Cat# D3922 |
|
Critical commercial assays |
|
Mouse CCL2 DuoSet ELISA |
R&D Systems |
Cat# DY479-05 |
Norepinephrine ELISA Kit |
Tebu-bio |
Cat# 157KA1891 |
Aldosterone Parameter Assay Kit |
R&D Systems |
Cat# KGE016 |
Corticosterone Parameter Assay Kit |
R&D Systems |
Cat# KGE009 |
Ki67 Staining Kit PE |
BD Biosciences |
Cat# 51-36525× |
FoxP3 Staining Buffer Set |
Miltenyi |
Cat# 130-093-142 |
RNA extraction kit |
QIAGEN |
Cat# 74136 |
|
Software and algorithms |
|
Prism 8 |
GraphPad |
N/A |
Chromeleon software |
Thermo Scientific |
N/A |
FlowJo |
Tree Star |
N/A |
Seurat package version 3.1.0 |
https://satijalab.org/seurat/ |
N/A |
BD FACSDiva |
BD Biosciences |
N/A |
ImageJ |
NIH |
N/A |
SpectroFlo |
Cytek |
N/A |
Phantasus |
Artyomov Lab |
N/A |