| Antibodies |
| Anti-Calnexin |
Sigma Aldrich |
C4731; RRID:AB_476845 |
| Anti-STAT6 |
R&D Systems |
AF2167; RRID:AB_355173 |
| Anti-pSTAT6 |
Sigma Aldrich |
SAB 4504546; RRID:AB_2827995 |
| Anti-STAT3 |
Thermofisher |
MA1-13042; RRID:AB_10985240 |
| Anti-pSTAT3 |
Thermofisher |
44-384G; RRID:AB_1502076 |
| Anti-pSTAT5 |
Sigma Aldrich |
SAB4301474; RRID:AB_2827996 |
| Anti-STAT5 |
Thermofisher |
PA5-36075; RRID:AB_1553359 |
| Anti-GAPDH |
Santa Cruz Biotechnology |
sc-32233; RRID:AB_627679 |
| Anti-Periostin |
Thermofisher |
PA5-34641; RRID:AB_2551993 |
| Anti-TSLPR |
ProSci |
4209; RRID:AB_10904285 |
| Anti-ITGB3 |
Novus Biological |
NBP1-60872; RRID:AB_11005987 |
| Goat Anti-Rabbit Cy3 |
Life Technologies |
A10520; RRID:AB_2534029 |
| Goat anti-rabbit IgG-HRP |
Santa Cruz Biotechnology |
sc-2004; RRID:AB_631746 |
| Goat anti-mouse IgG HRP |
Santa Cruz Biotechnology |
sc-2005; RRID:AB_631736 |
| Goat Anti-Mouse 488 |
Life Technologies |
A11001; RRID:AB_2534069 |
| Biological Samples |
| Mouse DRG |
This paper |
N/A |
| Mouse Skin |
This paper |
N/A |
| Mast cells |
Bone marrow (C57BL6J) |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
| Recombinant Mouse Periostin |
R&D Biosystems |
Catalog # 2955-F2 |
| Recombinant mouse IL-3 |
R&D Biosystems |
Catalog # 403-ML |
| Recombinant mouse SCF |
R&D Biosystems |
Catalog # 455-MC |
| Mouse anti-DNP IgE (clone SPE-7) |
Sigma Aldrich |
D8406 |
| DNP-HSA |
Sigma Aldrich |
A6661 |
| Ionomycin calcium salt |
Invitrogen |
I24222 |
| Dispase |
Fisher Scientific |
NC9886504 |
| Collagenase |
MP Biomedicals |
150704 |
| Laminin |
Sigma Aldrich |
L2020 |
| DMEM |
Atlanta Biologicals |
10-013-CV |
| RPMI-1640 |
Corning, NY |
10-040-CM |
| Pefabloc |
Sigma Aldrich |
15633 |
| FBS |
Atlanta Biologicals |
S11050 |
| PenStrep |
VWR |
K952 |
| Poly-L-Lysine |
Sigma Aldrich |
P4704 |
| Fura-2 AM |
Enzo |
ENZ-52006 |
| Recombinant Mouse TSLP |
R&D Biosystems |
555-TS |
| Histamine |
Sigma Aldrich |
H125 |
| Chloroquine |
Sigma Aldrich |
C6628 |
| Allyl isothiocyanate (AITC) |
Sigma Aldrich |
W203408 |
| Capsaicin |
Sigma Aldrich |
M2028 |
| Cilengitide |
Sigma Aldrich |
SMC1594 |
| Niclosamide |
Tocris Biosciences |
4079 |
| SD 1008 |
Tocris Biosciences |
3035 |
| MC903 |
Tocris Biosciences |
2700 |
| House Dust Mites (Dermatophagoides farina) |
Greer, Lenoir, NC, USA |
NC1563903 |
| Betamethasone dipropionate |
Sigma Aldrich |
5593 |
| Critical Commercial Assays |
|
In vivo jetPEI |
Polyplus |
201-10G |
| QIAGEN RNEasy Mini Kit |
QIAGEN |
74704 |
| Mouse Periostin/OSF-2 DuoSet ELISA |
R&D Biosystems |
DY2955 |
| Experimental Models: Cell Lines |
| Mouse Keratinocytes cell line (BALB/MK2 keratinocytes) |
This paper |
Gift from Dr. Marcelo Rodriguez, CVM/NCSU |
| Experimental Models: Organisms/Strains |
| WT: C57BL/6J |
Jackson Labs |
Strain Code: 000664 |
| ITGB3fl/fl: C57BL/6-Itgb3tm1.1Wlbcr/J |
Jackson Labs |
Strain Code: 028232 |
| SST-cre: B6J.Cg-Ssttm2.1(cre)Zjh/MwarJ |
Jackson Labs |
Strain Code: 028864 |
| TRPV1-cre: B6.129-Trpv1tm1(cre)Bbm/J |
Jackson Labs |
Strain Code: 017769 |
| SST-cre::Ai9 |
This Paper |
Strain Code: N/A |
| TRPV1-cre::β3−/− |
This Paper |
Strain Code: N/A |
| TRPV1 KO: B6.129X1-Trpv1tm1Jul/J |
Jackson Labs |
Strain Code: 003770 |
| TRPA1 KO: Trpa1tm2.1Kykw/J |
Jackson Labs |
Strain Code: 008654 |
| TRPV1/A1 Double Knockout |
This Paper |
Strain Code: N/A |
| NOD: NOD.CB17-Prkdcscid/J |
Jackson Labs |
Strain Code: 001303 |
| NOD/SCID: NOD/ShiLtJ |
Jackson Labs |
Strain Code: 001976 |
| Rag1 KO: B6.129S7-Rag1tm1Mom/J |
Jackson Labs |
Strain Code: 002216 |
| Ai9: B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J |
Jackson Labs |
Strain Code: 007909 |
|
KitW-sh/HNihrJaeBsmJ |
Jackson Labs |
005051 |
| NC/Nga |
Charles River Lab (Japan) |
RBRC01059 |
| Maltese-beagle |
in house |
CVM/NCSU |
| Non-Human Primates |
in house |
Wake Forest School of Medicine |
| Oligonucleotides |
| Taqman Mouse ITGAV Probe Set |
Thermofisher |
Mm01339538 |
| Taqman Mouse ITGB3 Probe Set |
Thermofisher |
Mm1240368 |
| Taqman Mouse ITGB5 Probe Set |
Thermofisher |
Mm00439825 |
| Taqman Mouse ITGA2b Probe Set |
Thermofisher |
Mm00439747 |
| Taqman Mouse GAPDH Probe Set |
Thermofisher |
Mm99999615 |
| Taqman Dog ITGAV Probe Set |
Thermofisher |
Cf02696697 |
| Taqman Dog ITGB3 Probe Set |
Thermofisher |
Cf02623437 |
| Taqman Dog ITGB5 Probe Set |
Thermofisher |
Cf02658863 |
| Taqman Dog ITGA2b Probe Set |
Thermofisher |
Cf02623652 |
| Taqman Dog GAPDH Probe Set |
Thermofisher |
Cf04419463 |
| Software and Algorithms |
| ImageJ |
NIH |
NA |
| Prism8 |
Graphpad |
NA |
| pClamp10 software |
Axon Instruments |
NA |
| NIS-Elements AR 5.02.01 |
Nikon |
NA |
| Other |
| Calcium Imaging Scope |
Nikon |
TE200 |
| Axopatch-700B amplifier |
Axon Instrument |
N/A |
| Eclipse Ti |
Nikon, Melville (NY) |
N/A |
| Dynamic Plantar Aesthesiometer |
Ugo Basile, Italy |
37450 |
| Rotarod Rotomex 5 |
Columbus Instruments |
N/A |
| Hargreave’s Apparatus |
Ugo Basile, Italy |
N/A |
| Cold Test |
Dry Ice assay |
Brenner et al., 2012
|
| BioTek Neo2 multimode plate reader |
BioTek |
N/A |
