TABLE 1.

Comparison of the relevant enzyme activities in Mycobacterium strain HL 4-NT-1 and P. pseudoalcaligenes JS45

Enzyme and substrate	Relative activity (sp act [μmol min−1 mg of protein−1])
	HL 4-NT-1a	JS45b
Dioxygenase
2-Aminophenol	17	100 (21.6)c
2-Amino-4-methylphenol	100 (0.13)	25c
Catechold	23	13c
4-Methylcatechol	NDe	NDc
Dehydrogenase
2-Aminomuconic semialdehyde	34	311
2-Amino-5-methylmuconic semialdehyde	112	9
2-Hydroxymuconic semialdehyde	100 (0.17)	100 (4.3)
2-Hydroxy-5-methylmuconic semialdehyde	111	32
Deaminase
2-Aminomuconate	100 (1.5)	100 (37.8)
2-Amino-5-methylmuconate	10	1.2
Decarboxylase
2-Oxo-3-hexene-1,6-dioate	100 (0.41)	100 (1.7)
5-Methyl-2-oxo-3-hexene-1,6-dioate	2.5	9.5

^aData were obtained with partially purified enzymes, except those for the dioxygenase activity, which were obtained with crude extracts. 

^bData were obtained with purified dioxygenase, dehydrogenase, and deaminase. Data for the decarboxylase activity were obtained with partially purified enzyme. 

^cData are from reference 17. 

^dCatechol was a suicide substrate. 

^eND, not detected.