A 1H anatomic imaging slice covering the heart (A) and the corresponding 2D 2H CSI spectra acquired from a representative rat before (baseline) (B) and 11.5 min (C), 23 min (D), and 34.5 min (E) after the [2,2,2-d3]-acetate infusion (approximately 1 min per CSI acquisition time), where the color code represents the acetate concentration of different voxels. Most acetate signals appear largely around the center of the heart and peaked at 23 min after the start of infusion. The green dash line and yellow/white dash circle represent the chest wall and heart region, respectively. The 1H/2H dual frequency RF surface coil was placed near the rat heart as indicated by a red oval-shaped circle. CSI, chemical shift imaging