KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
AAV2/8-Ef1a-Flex-GFP	Kim et al. (2013) https://doi.org/10.1523/JNEUROSCI.0035%5f13.2013	N/A
AAV2/8-Ef1a-CreN-InteinN	This Paper	N/A
AAV2/9-Ef1a-CreN-InteinN	This Paper	N/A
Retrograde AAV-Ef1a-InteinC-CreC	This Paper	N/A
Retrograde AAV-Ef1a-CreN-InteinN	This Paper	N/A
AAV2/9-hSyn-Cre	This Paper	N/A
AAV2/8-Ef1a-Flex-hChR2(H134R)-EYFP-WPRE-HGHpA	Purification using Addgene # 20298 plasmid Deposited by Karl Deisseroth (RRID: Addgene_20298)	N/A
pGP-AAV2/9-Syn-Flex-jGCaMP7f-WPRE	Addgene Dana et al., 2019 https://doi.org/10.1038/s41592-019-0435-6. Epub 2019 Jun 17	Addgene viral prep# 104492-AAV9
AAV DJ-Flex-EF1a-eArch3.0-EYFP	Stanford vector core https://doi.org/10.1038/nmeth.1808	GVVC-AAV-55
AAV9-hSyn-GFP	This Paper	N/A
Escherichia coli; Stbl3	Thermo Fisher	C737303
Chemicals, peptides, and recombinant proteins
DAPI	Sigma	10236276001
Isoflurane	Henry Schein	1182097
C&B Metabond® Quick Adhesive Cement System	Parkell	S380
Ethicon Suture 3/0	Ethicon	669H
Heparin sodium salt	Sigma	H3393
Triton X-100	IBI scientific	IB07100
Glycerol Proteomics Grade	VWR	VWRVM152-1L
Paraformaldehyde, PRILLS	Electron Microscopy Sciences	19202
Paraformaldehyde 32% solution, EM Grade	Electron Microscopy Sciences	15714-S
Tissue-Tek® O.C.T. Compound	Sakura	4583
Mounting medium: ProLong Glass	Invitrogen	P36980
PEI	Polyscience	24765
Benzonase	Novagen	70664
Phenol red	Sigma	P0290
Optiprep	Sigma	D1556
SHIELD Buffer solution	LifeCanvas Technologies	CAT #: SH-BS
SHIELD Epoxy solution	LifeCanvas Technologies	CAT #: SH-EX
SHIELD ON buffer	LifeCanvas Technologies	CAT #: SH-ON
SmartClear Clearing Buffer Type A & B	LifeCanvas Technologies	CAT #: SC-B2001
EasyIndex Optical Clearing Solution	LifeCanvas Technologies	CAT #: EI-Z1001
DMEM Nutrient mix F12	Gibco	11320082
Opti-MEM	Gibco	31985070
Trypsin 0.05% EDTA	Corning	25300054
Penicillin Streptomycin Solution	Gibco	15140122
Heat Inactivated Fetal Bovine Serum	Gibco	10438026
Cholrea toxin subunit B-Alexa Fluor 488 conjugated	Invitrogen	C22841
Tetrodotoxin citrate	Tocris	1069/1
Picrotoxin	Sigma	P1675
D-AP5	Tocris	0106/10
DNQX	Sigma	D0540-50MG
Experimental models: Cell lines
HEK293T	ATCC	CRL-11268
Experimental models: Organisms/strains
Mouse: C57BL/6J	The Jackson Laboratory	#000664
Mouse: Ai-14 (B6; 129S6-Gt (ROSA)26Sortm14(CAG-tdTomato)Hze/J)	The Jackson Laboratory	#007908
Mouse: Shank3 f/f(Δe4-9)	Yale University	N/A
Mouse: C3H/HeJ	The Jackson Laboratory	#000659
Recombinant DNA
AAV-Ef1a-Flex-GFP	Kim et al. (2013) https://doi.org/10.1523/JNEUROSCI.0035-13.2013	N/A
AAV-Ef1a-CreN-InteinN	This Paper	N/A
AAV-Ef1a-InteinC-CreC	This Paper	N/A
AAV-hSyn-Cre	This Paper	N/A
AAV-Ef1a-Flex-hChR2(H134R)-EYFP-WPRE-HGHpA	Addgene Deposited by Karl Deisseroth (RRID: Addgene_20298)	Addgene plasmid # 20298
AAV2/8	Addgene Deposited by James M. Wilson (RRID: Addgene_112864)	Addgene plasmid # 112864
AAV2/9	Addgene Deposited by James M. Wilson (RRID: Addgene_112865)	Addgene plasmid # 112865
Retrograde AAV	Addgene Tervo et al. (2016) https://doi.org/10.1016/j.neuron.2016.09.021	Addgene plasmid # 81070
pAd-deltaF	Addgene Deposited by James M. Wilson (RRID: Addgene_112867)	Addgene plasmid # 112867
Software and algorithms
Prism 9.0	GraphPad https://www.graphpad.com	N/A
SPSS 25	IBM	N/A
MATLAB R2020a	Mathworks https://www.ibm.com	N/A
Classification Learner ToolBox	Mathworks https://www.mathworks.com	N/A
Python 3.4	Python https://www.python.org/	N/A
Scikit-Learn package	Python https://scikit-learn.org/stable/	N/A
NetworkX package	Python https://networkx.org/	N/A
nVista Data Acquisition Software	Inscopix https://www.inscopix.com/	N/A
Inscopix Data Processing Software	Inscopix https://www.inscopix.com/	N/A
CNMFe	Pnevmatikakis et al. (2016) https://doi.org/10.1016/j.neuron.2015.11.037	N/A
EthoVisionXT	Noldus https://www.noldus.com/	N/A
Zen software	Zeiss https://www.zeiss.com/	N/A
SyGlass	Isto Visio RRID:SCR_017961	N/A
ImageJ	ImageJ https://imagej.nih.gov/ij/	N/A
SpineJ	Levet et al. (2020) https://doi.org/10.1016/j.ymeth.2020.01.020 https://github.com/flevet/SpineJ	N/A
Sketch	Arduino https://www.arduino.cc/	N/A
Inventor	Autodesk https://www.autodesk.com/	N/A
FMRIB software Library	Jenkinson et al. (2012) https://doi.org/10.1016/j.neuroimage.2011.09.015 https://www.fmrib.ox.ac.uk/datasets/techrep/tr04ss2/tr04ss2/node19.html	N/A
ICA-AROMA	Pruim et al. (2015) https://doi.org/10.1016/j.neuroimage.2015.02.064 https://github.com/maartenmennes/ICA-AROMA	N/A
MELODIC	FMRIB software Library Beckmann and Smith (2004) https://doi.org/10.1109/TMI.2003.822821	N/A
ANTs	Avants et al. (2011) https://doi.org/10.1016/j.neuroimage.2010.09.025 https://github.com/ANTsX/ANTs/	N/A
FAST	Zhang et al. (2001) FMRIB software Library https://doi.org/10.1109/42.906424	N/A
90 fROIs	Shirer et al. (2012) roi: https://doi.org/10.1093/cercor/bhr099 https://findlab.stanford.edu/functional_ROIs.html	N/A
AAL2 atlas	Rolls et al. (2015) https://doi.org/10.1016/j.neuroimage.2015.07.075 https://www.gin.cnrs.fr/en/	N/A
MVPA-Light	Treder (2020) https://doi.org/10.3389/fnins.2020.00289 https://github.com/treder/MVPA-Light	N/A
pCLAMP10	Molecular Devices
BioRender	https://biorender.com/	N/A
ABIDE dataset	Mind Research Network Collaborative informatics and neuroimaging suite (COINS) data exchange Landis et al. (2016) https://doi.org/10.1016/j.neuroimage.2015.05.049 https://coins.trendscenter.org/	N/A
Lindquist Dynamic Correlation Toolbox	https://github.com/canlab/Lindquist_Dynamic_Correlation/tree/master/DCC_toolbox	N/A
Deposited data
Round Social Arena	Zenodo: https://doi.org/10.5281/zenodo.6536336; https://zenodo.org/badge/latestdoi/333558926	N/A
Other
nVista	Inscopix https://www.inscopix.com/	N/A
Dual Channel Optogenetics-LED	Prizmatix https://www.prizmatix.com	N/A
Fiber-Optic Rotary Joint	Doric	FRJ_1 × 1_FC
Splitter Branching Fiber-Optic Patch Cords	Doric	N/A
Rotamex	Columbus Instruments http://www.colinst.com/	N/A
Oculus Rift S	Oculus https://www.oculus.com/	N/A
Mini USB IO Box	Noldus https://www.noldus.com/ethovision-xt/io-box	N/A
Arduino UNO	Arduino https://www.arduino.cc/	N/A
Basler Ace color camera with Gigabit Ethernet Interface	Basler https://www.baslerweb.com/	N/A
SVPRO Fisheye Lens 180 Degree USB Camera Module HD 1080P, Sony IMX322 USB Camera Board with Microphone	SVPRO	N/A
CELENA-S Digital Imaging System	Logos biosystems https://logosbio.com/	N/A
Zeiss LSM 710 Confocal Microscope	Zeiss https://www.zeiss.com/	LSM 710
Leica CM 1950 Cryostat	Leica https://www.leica-microsystems.com/	CM 1950
Vibratome 1000 Plus Sectioning System	Vibratome	N/A
Model 962 Dual Ultra Precise Small Animal Stereotaxic	David Kopf Instruments https://kopfinstruments.com/	Model 962
Nanoject II nanoliter injector	Drummond Scientific Company https://www.drummondsci.com/	N/A
V-1 Tabletop Active Scavenging Anesthesia Machine	VetEquip http://www.vetequip.com/	N/A
Model P-97 Flaming/brown micropipette puller	Sutter Instrument https://www.sutter.com/	P-97
Sorvall Legend X1 Centrifuge	Thermo Fisher	N/A
Sorvall Lynx 6000 Centrifuge	Thermo Fisher	N/A
Beckman L8-70M Ultracentrifuge	Beckman Coulter	N/A
Legend Micro21R Centrifuge	Thermo Fisher	N/A
1300 Series A2 Biosafety Cabinet	Thermo Fisher	Model 1385
Thermo Scientific CO2 Incubator	Thermo Fisher	Model 370
Isotemp Incubator	Thermo Fisher	151030513
SmartClear Pro II	LifeCanvas Technologies https://lifecanvastech.com	N/A
Form2 SLA 3D Printer	Formlabs https://formlabs.com	Form2
Dremel Digilab 3D20 3D Printer	Dremel Digilab https://digilab.dremel.com/	3D20
iQ5 Multicolor Real-Time PCR Detection System	BIO-RAD https://www.bio-rad.com	N/A
Leica VT1200S vibratome	Leica https://www.leica-microsystems.com/	VT1200S
CV-7B headstage	Molecular Devices	CV-7B
Multiclamp 700B	Molecular Devices	Multiclamp 700B
Digidata 1440A	Molecular Devices	Digidata 1440A
MPC-200	Sutter Instrument https://www.sutter.com/	MPC-200
ROE-200	Sutter Instrument https://www.sutter.com/	ROE-200
Olympus BX50WI Microscope		Olympus BX50WI
Advanced micro osmometer	Advanced Instruments. Inc.	Model 3300
Borosilicate Glass with Filament (O.D.: 1.2mm, I.D.: 0.94mm, 8cm length) for viral injection	Sutter Instrument https://www.sutter.com/	ITEM #: BF120-94-8
Borosilicate Glass Capillaries (O.D.: 1.5mm, I.D.: 1.12mm, 10cm length) for patch clamp	World precision instruments https://www.wpiinc.com/	TW150-4
Square Box Filament	Sutter Instrument https://www.sutter.com/	ITEM #: FB255B
ProView™ Lens Probe 1.0 diametter, ~4mm length	Inscopix https://www.inscopix.com/	1050-004605–IPN:100–001851
Formlabs Clear Resion V4	Formlabs https://formlabs.com	RS-F2-GPCL-04
White PLA Filament	Dremel Digilab https://digilab.dremel.com/	N/A
Fiber-optic probes (400mm fiber core diameter, 1.25mm OD ferrule, 0.5NA)	RWD Life Sciences Inc. https://www.rwdstco.com/	ITEM #: 807-00048-00
Amicon Ultra-15 Centrifugal Filters (100k MWCO)	Millipore Sigma	CAT #: UFC900308