FIG. 6.
Western blotting analysis of the extracellular protein production of strain MGG029(pMnp1.I)#25 cultured in differently supplemented AMM-maltodextrin media. −, no supplementation; Fe, FeSO4; APO, apohemoglobin; HEM, hemin; HMG, hemoglobin. (a) Production of MnP: blot was probed with an anti-MnP polyclonal antibody. (b) Production of amylase (AMY): blot was probed with a cross-reacting anti-GLA polyclonal antibody. (c) Band intensities of the MnP signals (RMnP) and amylase signals (RAMY), relative to the unsupplemented medium.