TABLE 2.
Sequences of the oligonucleotides used for cloning
| Oligonucleotide | Sequence |
|---|---|
| EcoRINcoI | |
| LipA5E/Na | GGAATTCCATGGCCTTCAAGCAG |
| MAFKQ | |
| BamHIHindIII | |
| Lip3B/Ha | CGGGATCCAAGCTTAAGCACCCGGAGG |
| StopA G P P | |
| EcoRINcoI | |
| MNP15E/Na | GGAATTCCATGGCCTTCGGTTCT |
| M A F G S | |
| BamHIHindII | |
| MNP13B/Ha | CGGGATCCAAGCTTAGGCAGGGCCATC |
| Stop A P G D | |
| EcoRI BbsI | |
| MNP1E/Ba | GCGAATTCGAAGACCTCGCGCAGTCTGTCCAGA |
| A V C P D | |
| BssHII | |
| OLIGONARAb | CGCGCTTGGAAATCACATT |
| R K S I V N | |
| NarI | |
| OLIGONARBb | CGAATGTGATTTCCAAG |
| N V I S K |
a
Used as primer for PCR.
b
Used to construct the linker NarI-NVISKR-BssHII.