TABLE 3.
Extracellular rMnP production of strain MGG029(pMn1.I)#25 after 36 h of growth in differently supplemented AMM-maltodextrin media
| Medium | Activity (ΔAbs/min/ml)a | Extracellular rMnP (mg/liter)c |
|---|---|---|
| AMM-maltodextrin | 6.0 ± 0.8b | 9.6 ± 1.2 |
| AMM-maltodextrin + 0.02 g/liter of FeSO4 | 6.7 ± 0.9 | 10.7 ± 1.4 |
| AMM-maltodextrin + 500 mg/liter of hemin | 41.8 ± 0.8 | 66.9 ± 1.2 |
| AMM-maltodextrin + 5 g/liter of apohemoglobin | 19.7 ± 0.6 | 31.5 ± 0.9 |
| AMM-maltodextrin + 5 g/liter of hemoglobin | 66.2 ± 12.1 | 105.9 ± 19.4 |
a
Activity was measured by ABTS oxidation (see Materials and Methods), and protein values were calculated based on MnP specific activity.
b
The values presented are the averages of at least two measurements in two different experiments, ± standard deviations.
c
Values are means ± standard deviations.