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. Author manuscript; available in PMC: 2022 Jun 21.
Published in final edited form as: DNA Repair (Amst). 2020 Jul 10;94:102926. doi: 10.1016/j.dnarep.2020.102926

Fig. 5. Repair of TOP-DPC by the non-proteasomal metalloproteases SPRTN/Wss1.

Fig. 5.

a. Domain/motif architecture of human SPRTN and its yeast ortholog Wss1. ZBD, Zinc binding domain; PIP, PCNA interaction peptide; SHP, p97 or VCP-binding motif; SIM, SUMO interaction motif; SprT, the metalloprotease domain similar to that of the Escherichia coli SprT protein; UBZ, ubiquitin-binding zinc finger; VIM; VCP interaction motif; WLM, Wss1- like metalloprotease domain Schematic of the repair of TOP-DPC by SPRTN/Wss1. b. As a component of the replisome, SPRTN/WSS1 is activated by its binding to ssDNA. It targets TOP-DPC as well as general DPC for proteolysis in association with replication.