. Author manuscript; available in PMC: 2022 Jun 21.

Published in final edited form as: DNA Repair (Amst). 2020 Jul 10;94:102926. doi: 10.1016/j.dnarep.2020.102926

Table 2.

Ubiquitin ligases for proteasomal degradation of TOP-DPC.

Ubiquitin Ligase	Detection/signal	Substrate	TOP-DPC Inducing Agent	References
Cullin 3-RING ligase 3 (CRL3)	Unknown	TOP1-DPC	CPT	[67]
BRCA1	Transcription and serine 10 phosphorylation of TOP1 by DNA-PK	TOP1-DPC	CPT	[77,235]
Cullin 4B-RING ligase (CRL4B)	Unknown	TOP1-DPC	CPT	[76]
Slx5-Slx8 heterodimer	SUMOylation in yeast S. cerevisiae by Siz1	TOP1- and TOP2-DPC	CPT, etoposide and amsacrine	[68,119,236]
RNF4 homodimer (Slx5-Slx8 ortholog)	SUMOylation in human by PIAS4	TOP1- and TOP2-DPC	CPT and etoposide	[68]
SCF^β-TrCP (SKP1-Cullin 1-F box protein (β-TrCP)	Phosphorylation of TOP2β in its degron motif	TOP2β-DPC	Teniposide (VM26)	[123]
Bmi1-Ring1A Heterodimer	Unkown	TOP2α-DPC	Teniposide (VM26)	[85]