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. 2022 Jun 20;19:162. doi: 10.1186/s12974-022-02525-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Effects of DREADD-hM3Dq and hM4Di on ACC CX3CL1 protein levels and the mechanical paw withdrawal thresholds. A Behavioral test paradigms are shown on the top and representative western blotting for bilateral ACC CX3CL1 in the mice injected with DREADD-Gq or DREADD-Gi are shown below. B The injection site of virus carrying hM3Dq-, hM4Di-mCherry or mCherry in contralateral ACC. C Quantification of bilateral CX3CL1 in the mice injected with DREADD-Gq and DREADD-Gi, respectively. *p < 0.05, ***p < 0.001 versus mCherry control groups (n = 3 mice/group, two-way ANOVA). D Compared to the mCherry control mice, DREADD-hM3Dq induced significant decrease of threshold in ipsilateral hind paws of normal mice (left); DREADD-hM4Di reversed the SNI-induced decrease of ipsilateral threshold (right). *p < 0.05 versus mCherry control groups (n = 6 mice/group, multiple t tests)