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. 2022 Jun 21;20:291. doi: 10.1186/s12951-022-01504-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — miR-762 and iPSCs-KCs-Exos promote migration of keratinocyte and endothelial cells by targeting PML. A Predicted miR-762 target sequences in PML 3’-UTR and the mutated nucleotides in the 3′UTR of PML. B The relative luciferase activity in 293 T cells with psiCHECK-PML-wt-3′UTR (WT) or psiCHECK-PML-mut-3’UTR (MUT) and miR-762 mimics co-transfection was evaluated by luciferase reporter assay. C, D qRT–PCR and Western blot analysis of PML expression in HaCaT cells and HUVECs which were treated with control, miR-762 mimics and iPSCs-KCs-Exos (Exos). E Representative images of the wound healing assay and quantitative analysis of the wound healing rates of HaCaT cells, which were treated with a control, b miR-762 mimics, c iPSCs-KCs-Exos (Exos), d pcDNA3.1-PML (PML), e miR-762 mimic plus pcDNA3.1-PML (PML), f iPSCs-KCs-Exos (Exos) plus pcDNA3.1-PML (PML). Scale bar, 200 μm. F Images of migrated HUVECs and quantitative analysis of the migrated cells, which were treated with a control, b miR-762 mimics, c iPSCs-KCs-Exos (Exos), d pcDNA3.1-PML (PML), e miR-762 mimic plus pcDNA3.1-PML (PML), f iPSCs-KCs-Exos (Exos) plus pcDNA3.1-PML (PML). Scale bar, 200 μm. *P < 0.05, **P < 0.01