Fig. 5.

PMLA-based nanoparticles for tumor theranostics. a (i) MRI brain scans of mice with double tumors: a GBM (U87MG, EGFR⁺) in the left hemisphere and a metastatic breast cancer (BT-474, HER2⁺) in the right hemisphere after IV injection of (ii) targeted PMLA conjugated with Gd-DOTA/cetuximab/mouse TfR-mAb/Alexa 680, with (iii) quantitative analysis of MRI contrast in tumors and (iv) confirmation of MRI diagnosis by immunohistochemical analysis. Reprinted from ref. [106], with permission from the American Chemical Society, Copyright 2015. b (i) MRI scans of mice with double tumors, a primary GBM (U87MG, EGFR⁺) in the left hemisphere and metastatic breast cancer (BT-474, HER2⁺) in the right hemisphere after IV injection of (ii) targeted PMLA conjugated with Gd-DOTA/trastuzumab/mouse TfR-mAb/Alexa 680, with (iii) quantitative analysis of MRI contrast in tumors and (iv) confirmation of MRI diagnosis by immunohistochemical analysis. Reprinted from ref. [106], with permission from the American Chemical Society, Copyright 2015. c Schematic presentation of (i) PMLA-based nanodrugs, (ii) stereotactic implantation of brain tumors, and (iii) proposed mechanism of action. (iv) Kaplan–Meier animal survival curve for treatment of HER2⁺ BT-474 brain metastasis, EGFR⁺ A-549 brain metastasis, EGFR⁺ triple-negative MDA-MB-468 brain metastasis. The corresponding PMLA nanoconjugates improved survival by 57%, 66%, and by 114%, respectively (from left to right). Reprinted from ref. [106], with permission from the American Chemical Society, Copyright 2015