Fig. 4.
Clarithromycin downregulates inflammatory gene expression in the placenta. A Dams were intra-amniotically injected with HMGB1 under ultrasound guidance on 14.5 days post coitum (dpc) and treated with 75 mg/kg of clarithromycin (CLR; n = 7) or DMSO (vehicle control; n = 8) at 6, 12, 24, 48, 72, and 96 h post-injection. On 18.5 dpc, two hours after the last dose of CLR or DMSO, mice were euthanized and tissue collection was performed to obtain the placenta and assess inflammatory gene expression. Expression (-ΔCT) of B Il6, C Tnf, D Il12b, E Ccl3, F Ccl5, G Ccl22, H Cxcl9, I Cxcl10, J Tlr9, and K Nod1 from dams intra-amniotically injected with HMGB1 and treated with CLR (blue dots) or DMSO (red dots). Data are represented as box-and-whisker plots with medians, interquartile ranges, and min/max ranges. P-values were determined using the one-sided Mann–Whitney U-test