TABLE 1.
Adhesion and penetration of V. shiloi onto and into O. patagonicaa
Time after infection (h) | % Adhesionb ± SE | % Penetrationc ±SE |
---|---|---|
4 | 36 ± 10 | 0.9 ± 0.5 |
8 | 83 ± 0 | 5.6 ± 2.0 |
12 | 77 ± 16 | 20.9 ± 8.5 |
24 | 86 ± 7 | 21.7 ± 7.6 |
48 | 100 ± 0 | 5.3 ± 4.3 |
72 | 100 ± 0 | 0.0 ± 0 |
An overnight culture of V. shiloi was inoculated into 125-ml flasks containing healthy pieces of coral in 25 ml of sterile seawater. The flasks were incubated at 29°C with gentle shaking (80 rpm) with cycles alternating 12 h of light and 12 h of darkness. Data are averages from three experiments, each performed in duplicate with initial bacterial concentrations of ca. 4 × 106 per ml.
Calculated from CFU lost from the seawater.
Calculated from the CFU in the crushed coral (compared to the inoculated bacteria) following treatment of the coral with methyl-β-d-galactopyranoside and gentamicin.