TABLE 2.

Sensitivity of free and intracellular V. shiloi to treatment with gentamicin and methyl-β-d-galactopyranoside^a

Source of V. shiloi	CFU	Microscopic countb (total)
Coral mucus
Treated	<102	1.3 × 106
Untreated	1.1 × 106	1.0 × 106
Coral tissue (intracellular)
Treated	6.6 × 104	1.0 × 108
Untreated	3.4 × 104	1.4 × 108

^aThe experiment was performed as described in the legend to Fig. 3 with an initial inoculum of 2.6 × 10⁵ V. shiloi bacteria per ml. After incubation for 36 h, the corals were removed from the flasks and were shaken for 3 h in sterile seawater in either the presence (treated) or the absence (untreated) of gentamicin and methyl-β-d-galactopyranoside. Total microscopic counts and CFU were then determined on the treated and untreated samples of the free bacteria (from coral mucus) and the crushed coral tissue (intracellular bacteria). 

^bMicroscopic counts were determined by using specific anti-V. shiloi antibodies.