Fig. 4.

Tideglusib promoted PI3K/Akt phosphorylation in EpiSCs. a The proliferation effect and toxicity of different concentrations of Tideglusib on EpiSCs were compared by the SRB assay and CCK-8 experiment. *P < 0.05, **P < 0.01 vs. the control group. N = 6. b The phosphorylation of Akt and mTOR, the proliferation related protein Cyclin D1, the apoptosis related protein Bax and Bcl2 were assessed by Western Blot. N = 3 for each group. *P < 0.05, **P < 0.01 vs. the control group. c and d Flow cytometry was further used to quantify cell cycle distribution and apoptosis in cells treated with PBS or 200 nM Tideglusib. *P < 0.05, **P < 0.01 versus the control group. N = 3 for each group. e Confluent monolayers of EpiSCs were randomly divided into two groups that received PBS or 200 nM Tideglusib and subjected to in vitro scratch-wound assays. Quantifications of wound closure are shown in the graph right. N = 3 for each group. *P < 0.05, **P < 0.01 versus the control group at time-point