Fig. 3.

Antcin K inhibits VCAM-1 expression and monocyte adhesion in human RASFs. (a, b) Antcin K (0.3–10 μM) was administered to RASFs for 24 h, and then VCAM-1 expression was examined using RT-qPCR (n = 4) and Western blot analyses (n = 3). (c, d) RASFs were incubated with indicated concentrations of Antcin K for 24 h. BCECF-AM-labeled THP-1 cells were then added to the RASFs for 1 h. Monocyte adherence in cultured RASFs was examined by fluorescence microscopy and photographed (n = 4). *P < 0.05 compared with the control.