Table 1.

Primer, RCA template, and spacer complementary sequences designs. The RCA template which is composed of the complementary sequences of the anti-ST and anti-SE aptamers[13] is flanked primer-binding sites and spacer region. Of course, the RCA template was ordered as phosphorylated to facilitate the T4 DNA ligase (NEB, Cat# M0202S). The primer was designed to span the primer-binding sites of the two extremities of the RCA template.

Oligo	Sequence (5’→3’)
Primer	GTTCAGATGCAAGACGTTCC
Spacer	GATCCACCGGTAGCA
RCA template	5Phos/GCATCTGAAC [AATCGCTGAATGAAATCCACGTGCGGGACAGAGGCAGTGCGTGGCGTGCGGTGAGAGGTGGATTGTGCCTC GCGAATCTGTCCTGGACTG] TGCTACCGGTGGATC [AATCGCTGAATGAAATCCACGTGGATAGCGATGTTGATAGAGCGTGAGTTGGTCGTGTTTGGAGTGGGCT CGCGAATCTGTCCTGGACTG] GGAACGTCTT

RCA=Rolling circle amplification