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RING assay shows that only the NMDAR1F654A allele affects the capacity to climb. Age-matched female flies are knocked to the bottom of a vial, and naturally climb toward the top of the container. This is repeated five times and the height climbed by each fly is measured. The EP331 (Panel A; n.s. p > 0.5) and K558Q (Panel C; n.s. p > 0.1) alleles do not affect climbing (p > 0.05), while the F654A allele (Panel B; p < 0.03) of the NMDAR1 gene reduces climbing ability (p < 0.03).
