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. Author manuscript; available in PMC: 2022 Jun 21.
Published in final edited form as: Toxicol Sci. 2020 Feb 1;173(2):280–292. doi: 10.1093/toxsci/kfz227

Figure 3. In vitro and ex vivo TPO inhibition determined by AUR-TPO inhibition.

Figure 3.

Assay with MMI and PTU in a 96-well format. A) In vitro assay was performed with PTU on rat thyroid microsomes of non-treated rats exposed in vitro to 0–100 μM PTU N=6. IC50 = 1.21μM. B) TPO activity was inhibited at all doses in the ex vivo microsomes obtained from thyroid gland of 4-day PTU exposed animals. C) TPO inhibition measured from MMI exposed rat thyroid microsome in vitro IC50 = 0.11μM. D) TPO inhibition was also demonstrated at all doses in ex vivo microsomes from thyroid gland of 4-day MMI treated animals N=4 Z’ > 0.94. * represent p<0.05, and error bars ± SEM