TABLE 1.
Effect of CRISPRi-silencing wal on sensitivity to antibioticsa
| Antibiotic | MIC (μg/mL) |
||
|---|---|---|---|
| sgRNA-neg | sgRNA-walA1 | sgRNA-walA2 | |
| Ampicillin | 6.3 | 3.5 | 3.1 |
| Imipenem | 3.1 | 3.1 | 2.6 |
| Vancomycin | 4.6 | 0.9 | 0.7 |
| Daptomycin | 11.3 | 4.1 | 5.7 |
| Bacitracin | 250 | 250 | 250 |
| Novobiocin | 13 | 17 | 16 |
Results are the averages of measurements done in duplicate on four different days.