TABLE 4.
Strains used in this study
| Strain | Genotype and/or description | Source or reference(s) |
|---|---|---|
| E. coli | ||
| OmniMAX-2 T1R | F′ [proAB+ lacIq lacZΔM15 Tn10 (Tetr) Δ(ccdAB)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80(lacZ)ΔM15 Δ(lacZYA-argF) U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD | Invitrogen |
| HB101/pRK24 | F− mcrB mrr hsdS20(rB− mB−) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 | 66, 67 |
| C. difficile | ||
| R20291 | Wild-type C. difficile strain from UK outbreak (ribotype 027) | |
| 630Δerm | Spontaneous erythromycin-sensitive derivative of strain 630 (ribotype 012) | 76 |
| CRG1496 | 630Δerm ΔpyrE | 29 |
| UM275 | 630Δerm with Pveg::rfp downstream of pyrE | 26 |
| UM554 | 630Δerm with Pxyl::dCas9 Pgdh::sgRNA-neg downstream of pyrE | This study |
| UM555 | 630Δerm with Pxyl::dCas9 Pgdh::sgRNA-walA-1 downstream of pyrE | This study |
| UM556 | 630Δerm with Pxyl::dCas9 Pgdh::sgRNA-walA-2 downstream of pyrE | This study |
| UM626 | 630Δerm with Pxyl::walR downstream of pyrE | This study |
| UM628 | 630Δerm with Pxyl::walRD54E downstream of pyrE | This study |
| UM926 | 630Δerm with Pcd630_0739::mCherryOpt downstream of pyrE | This study |