TABLE 5.
Plasmids used in this study
| Plasmid | Relevant features | Reference or comment |
|---|---|---|
| pAP114 | Pxyl::mCherryOpt catP | 26 |
| pBZ101 | Pxyl empty vector | This study |
| pCE636 | PdltD::mCherryOpt catP | This study |
| pCE738 | Pxyl::dCas9-opt Pgdh::sgRNA-dltD-1 catP | 37 |
| pCE691 | Pxyl::walR catP | This study |
| pCE738 | Pxyl::dCas9-opt Pgdh::sgRNA-dltD-1 catP | 37 |
| pCE741 | Pxyl::cdr2656 | This study |
| pCE744 | Pxyl::dCas9-opt Pgdh::sgRNA-cd630_27680-1 catP | This study |
| pCE745 | Pxyl::dCas9-opt Pgdh::sgRNA-cd630_27680-2 catP | This study |
| pCE789 | Pxyl::dCas9-opt Pgdh::cwpV-1 catP | This study |
| pCE791 | Pxyl::cwpV | This study |
| pDSW1728 | Ptet::mCherryOpt catP | 55 |
| pDSW2037 | Pxyl in integration vector pMTL-YN1C catP | This study |
| pDSW2053 | Pxyl::dCas9opt-Pgdh::sgRNA-neg in pMTL-YN1C catP | This study |
| pDSW2055 | Pxyl::dCas9opt-Pgdh::sgRNA-cd630_17810-1 in pMTL-YN1C catP | This study |
| pDSW2057 | Pxyl::dCas9opt-Pgdh::sgRNA-cd630_17810-2 in pMTL-YN1C catP | This study |
| pIA33 | Pxyl::dCas9-opt Pgdh::sgRNA-rfp catP | 26 |
| pIA34 | Pxyl::dCas9-opt Pgdh::sgRNA-neg catP | 26 |
| pIA50 | Pxyl::dCas9-opt Pgdh::sgRNA-walA-1 catP | This study |
| pIA51 | Pxyl::dCas9-opt Pgdh::sgRNA-walA-2 catP | This study |
| pIA75 | Pxyl::walR in pMTL-YN1C catP | This study |
| pIA76 | Pxyl::walRD54E in pMTL-YN1C catP | This study |
| pIA79 | Pxyl::dCas9-opt Pgdh::cd630_25040-1 catP | This study |
| pIA80 | Pxyl::dCas9-opt Pgdh::cd630_25040-2 catP | This study |
| pIA81 | Pxyl::dCas9-opt Pgdh::cd630_36010-1 catP | This study |
| pIA93 | Pcdr_0665::mCherryOpt catP | This study; cdr_0665 is ortholog of cd630_07380 |
| pIA95 | Pcdr_0796::mCherryOpt catP | This study; cdr_0796 is ortholog of cd630_08670 |
| pIA97 | Pcdr_2753::mCherryOpt catP | This study; cdr_2753 is ortholog of cd630_28620 |
| pIA98 | Pcdr_0455::mCherryOpt catP | This study; cdr_0455 is ortholog of cd630_53000 |
| pIA100 | Pcd630_0739::mCherryOpt catP | This study |
| pIA101 | Pcd630_0739::mCherryOpt in pMTL-YN1C catP | This stduy |
| pIA102 | Pxyl::dCas9-opt Pgdh::cd630_05490 catP | This study |
| pIA103 | Pxyl::dCas9-opt Pgdh::cd630_27940 catP | This study |
| pIA104 | Pxyl::dCas9-opt Pgdh::cd630_27860 catP | This study |
| pIA105 | Pxyl::dCas9-opt Pgdh::cd630_27910 catP | This study |
| pIA106 | Pxyl::dCas9-opt Pgdh::cd630_10360 catP | This study |
| pIA107 | PpgdA::mCherryOpt catP | This study |
| pIA108 | Pxyl::dCas9-opt Pgdh::cd630_07380-1 catP | This study |
| pIA109 | Pxyl::dCas9-opt Pgdh::cd630_07380-2 catP | This study |
| pIA110 | Pxyl::dCas9-opt Pgdh::cd630_07390-1 catP | This study |
| pIA111 | Pxyl::dCas9-opt Pgdh::cd630_07390-2 catP | This study |
| pIA112 | Pxyl::cd630_0738 | This study |
| pIA113 | Pxyl::cd630_0739 | This study |
| pIA114 | Pxyl::cd630_0738-cd630_0739 | This study |
| pIA115 | Pxyl::cd630_0739-cd630_0738 | This study |
| pMTL-YN1C | E. coli-C. difficile shuttle vector for inserting genes into C. difficile chromosome while restoring pyrE; colE1 RP4oriT-TraJ CB102ori-repH′ catP | 29 |